通过表达谱变化分析监测两性霉素 B、伏立康唑和米卡芬净对烟曲霉的抗真菌活性。
Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus.
机构信息
Public Health Research Institute Center, UMDNJ-New Jersey Medical School, Newark, New Jersey, USA..
出版信息
Antimicrob Agents Chemother. 2012 May;56(5):2770-2. doi: 10.1128/AAC.06163-11. Epub 2012 Feb 6.
Abstract
Eight highly expressed candidate genes were selected for mRNA profiling to monitor the transcriptome kinetics of Aspergillus fumigatus strains exposed to antifungal drugs as potential biomarkers of live cells to assess treatment efficacy. Mycelia were treated with fungicidal drugs amphotericin B and voriconazole, as well as the fungistatic drug micafungin. Transcription was monitored at 0, 4, 8, and 24 h posttreatment. The expression turnover profile provides a possible tool to assess antifungal therapy effects.
摘要
选择了 8 个高表达的候选基因进行 mRNA 谱分析，以监测暴露于抗真菌药物的烟曲霉菌株的转录组动力学，作为活细胞的潜在生物标志物来评估治疗效果。用杀真菌药物两性霉素 B 和伏立康唑以及抑菌药物米卡芬净处理菌丝体。在处理后 0、4、8 和 24 小时监测转录。表达周转率谱提供了一种评估抗真菌治疗效果的可能工具。
相似文献
1
Expression turnover profiling to monitor the antifungal activities of amphotericin B, voriconazole, and micafungin against Aspergillus fumigatus.通过表达谱变化分析监测两性霉素 B、伏立康唑和米卡芬净对烟曲霉的抗真菌活性。
Antimicrob Agents Chemother. 2012 May;56(5):2770-2. doi: 10.1128/AAC.06163-11. Epub 2012 Feb 6.
2
In Vitro Interactions between Target of Rapamycin Kinase Inhibitor and Antifungal Agents against Aspergillus Species.雷帕霉素激酶抑制剂靶点与抗真菌药物对曲霉菌种的体外相互作用
Antimicrob Agents Chemother. 2016 May 23;60(6):3813-6. doi: 10.1128/AAC.02921-15. Print 2016 Jun.
3
Amphotericin B- and voriconazole-echinocandin combinations against Aspergillus spp.: Effect of serum on inhibitory and fungicidal interactions.两性霉素 B 和伏立康唑与棘白菌素联合治疗曲霉菌属：血清对抑制和杀菌相互作用的影响。
Antimicrob Agents Chemother. 2013 Oct;57(10):4656-63. doi: 10.1128/AAC.00597-13. Epub 2013 Jul 15.
4
Phase-dependent antifungal activity against Aspergillus fumigatus developing multicellular filamentous biofilms.对形成多细胞丝状生物膜的烟曲霉的阶段依赖性抗真菌活性。
J Antimicrob Chemother. 2008 Dec;62(6):1281-4. doi: 10.1093/jac/dkn402. Epub 2008 Sep 26.
5
A comparative study of fungicidal activities of voriconazole and amphotericin B against hyphae of Aspergillus fumigatus.伏立康唑与两性霉素B对烟曲霉菌丝杀菌活性的比较研究
J Antimicrob Chemother. 2005 Jun;55(6):914-20. doi: 10.1093/jac/dki100. Epub 2005 Apr 11.
6
In vitro antifungal combination effects of micafungin with fluconazole, voriconazole, amphotericin B, and flucytosine against clinical isolates of Candida species.米卡芬净与氟康唑、伏立康唑、两性霉素B和氟胞嘧啶对念珠菌属临床分离株的体外抗真菌联合作用。
J Infect Chemother. 2009 Feb;15(1):1-5. doi: 10.1007/s10156-008-0653-9. Epub 2009 Mar 12.
7
The activity of echinocandins, amphotericin B and voriconazole against fluconazole-susceptible and fluconazole-resistant Brazilian Candida glabrata isolates.棘白菌素类、两性霉素 B 和伏立康唑对氟康唑敏感和氟康唑耐药巴西光滑念珠菌分离株的活性。
Mem Inst Oswaldo Cruz. 2012 May;107(3):433-6. doi: 10.1590/s0074-02762012000300022.
8
In vitro efficacy of the combination of voriconazole and anidulafungin against voriconazole-resistant cyp51A mutants of Aspergillus fumigatus.烟曲霉唑康唑耐药 CYP51A 突变株的体外联合伏立康唑和安尼拉芬净的疗效。
Diagn Microbiol Infect Dis. 2012 Jun;73(2):135-7. doi: 10.1016/j.diagmicrobio.2012.02.003.
9
Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs.扫描四极杆数据非依赖性采集，第二部分：在唑类和棘白菌素类抗真菌药物治疗烟曲霉过程中分析钙调神经磷酸酶相互作用蛋白的应用。
J Proteome Res. 2018 Feb 2;17(2):780-793. doi: 10.1021/acs.jproteome.7b00499. Epub 2017 Dec 29.
10
A comparative study of the post-antifungal effect (PAFE) of amphotericin B, triazoles and echinocandins on Aspergillus fumigatus and Candida albicans.两性霉素B、三唑类和棘白菌素对烟曲霉和白色念珠菌的抗真菌后效应（PAFE）的比较研究。
J Antimicrob Chemother. 2004 Feb;53(2):386-9. doi: 10.1093/jac/dkh066. Epub 2004 Jan 16.
引用本文的文献
1
Tailoring amphotericin B as an ionic liquid: an upfront strategy to potentiate the biological activity of antifungal drugs.将两性霉素B定制为离子液体：增强抗真菌药物生物活性的前期策略。
RSC Adv. 2021 Apr 16;11(24):14441-14452. doi: 10.1039/d1ra00234a. eCollection 2021 Apr 15.
2
Transcriptomics Analysis of the Chinese Pear Pathotype of Gives Insights into Novel Mechanisms of HSAF Antifungal Activities.转录组分析揭示中国梨品种抗梨火疫病新机制
Int J Mol Sci. 2018 Jun 22;19(7):1841. doi: 10.3390/ijms19071841.
3
Antifungal drug testing by combining minimal inhibitory concentration testing with target identification by gas chromatography-mass spectrometry.通过最小抑菌浓度测试与气相色谱-质谱联用进行目标鉴定来进行抗真菌药物检测。
Nat Protoc. 2017 May;12(5):947-963. doi: 10.1038/nprot.2017.005. Epub 2017 Apr 6.
本文引用的文献
1
Aspergillus PCR: one step closer to standardization.曲霉 PCR：更接近标准化一步。
J Clin Microbiol. 2010 Apr;48(4):1231-40. doi: 10.1128/JCM.01767-09. Epub 2010 Feb 10.
2
Post-antifungal effect of amphotericin B and voriconazole against germinated Aspergillus fumigatus conidia.两性霉素B和伏立康唑对烟曲霉分生孢子萌发后的抗真菌作用。
J Antimicrob Chemother. 2008 Jun;61(6):1309-11. doi: 10.1093/jac/dkn129. Epub 2008 Mar 26.
3
Fungal infections in recipients of hematopoietic stem cell transplants: results of the SEIFEM B-2004 study--Sorveglianza Epidemiologica Infezioni Fungine Nelle Emopatie Maligne.造血干细胞移植受者的真菌感染：SEIFEM B - 2004研究结果——恶性血液病真菌性感染的流行病学监测
Clin Infect Dis. 2007 Nov 1;45(9):1161-70. doi: 10.1086/522189. Epub 2007 Sep 26.
4
Aspergillus infection in lung transplant patients: incidence and prognosis.肺移植患者的曲霉感染：发病率与预后
Eur J Clin Microbiol Infect Dis. 2007 Dec;26(12):879-86. doi: 10.1007/s10096-007-0376-3.
5
Invasive aspergillosis: epidemiology, diagnosis and management in immunocompromised patients.侵袭性曲霉病：免疫功能低下患者的流行病学、诊断与管理
Drugs. 2007;67(11):1567-601. doi: 10.2165/00003495-200767110-00004.
6
Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole.伏立康唑作用下烟曲霉的转录组分析
Curr Genet. 2006 Jul;50(1):32-44. doi: 10.1007/s00294-006-0073-2. Epub 2006 Apr 19.
7
Characterization of Aspergillus fumigatus mutants with reduced susceptibility to caspofungin.对卡泊芬净敏感性降低的烟曲霉突变体的鉴定
Med Mycol. 2005 May;43 Suppl 1:S299-305. doi: 10.1080/13693780400029023.
8
A comparative study of the post-antifungal effect (PAFE) of amphotericin B, triazoles and echinocandins on Aspergillus fumigatus and Candida albicans.两性霉素B、三唑类和棘白菌素对烟曲霉和白色念珠菌的抗真菌后效应（PAFE）的比较研究。
J Antimicrob Chemother. 2004 Feb;53(2):386-9. doi: 10.1093/jac/dkh066. Epub 2004 Jan 16.
9
Invasive aspergillosis in allogeneic stem cell transplant recipients: changes in epidemiology and risk factors.异基因干细胞移植受者中的侵袭性曲霉病：流行病学和危险因素的变化
Blood. 2002 Dec 15;100(13):4358-66. doi: 10.1182/blood-2002-05-1496. Epub 2002 Aug 22.
10
Method for measuring postantifungal effect in Aspergillus species.测量曲霉属真菌抗真菌后效应的方法。
Antimicrob Agents Chemother. 2002 Jun;46(6):1960-5. doi: 10.1128/AAC.46.6.1960-1965.2002.
Zotero 插件