Shelton K, Parkinson T J, Hunter M G, Kelly R W, Lamming G E
AFRC Research Group on Hormones and Farm Animal Reproduction, University of Nottingham, Loughborough, Leics, UK.
J Reprod Fertil. 1990 Sep;90(1):11-7. doi: 10.1530/jrf.0.0900011.
Heifers slaughtered on Day 18/19 of pregnancy had significantly higher (P less than 0.001) concentrations of PGE-2 (measured as its methyl oxime) in uterine flushings than did animals slaughtered on Days 6 or 12 of pregnancy, or on Days 6 or 12 of the oestrous cycle. In addition, concentrations were higher in the uterine horn ipsilateral to the corpus lueum on Days 12 (P less than 0.05) and 18/19 (P less than 0.01) than in the contralateral horn. Incubation of dispersed luteal cells for 3 h with LH (0.1 or 100 ng/ml) and/or PGE-2 (0.01-1000 ng/ml) in vitro showed no differences in basal progesterone production or in the responses to exogenous hormones between pregnant and non-pregnant cattle. However, low doses of PGE-2 (0.01-10 ng/ml) inhibited the stimulation of progesterone secretion by the lower dose of LH. These findings indicate that although PGE-2 can stimulate progesterone synthesis by luteal cells it may also have inhibitory effects, and therefore its role in pregnancy requires further definition.
怀孕第18/19天屠宰的小母牛子宫冲洗液中PGE - 2（以其甲基肟形式测量）的浓度显著高于（P小于0.001）怀孕第6天或12天、发情周期第6天或12天屠宰的动物。此外，在第12天（P小于0.05）和18/19天（P小于0.01），黄体同侧子宫角中的浓度高于对侧子宫角。体外将分散的黄体细胞与LH（0.1或100 ng/ml）和/或PGE - 2（0.01 - 1000 ng/ml）孵育3小时，结果显示怀孕和未怀孕母牛之间基础孕酮分泌或对外源激素的反应没有差异。然而，低剂量的PGE - 2（0.01 - 10 ng/ml）抑制了低剂量LH对孕酮分泌的刺激。这些发现表明，尽管PGE - 2可以刺激黄体细胞合成孕酮，但它也可能具有抑制作用，因此其在妊娠中的作用需要进一步明确。
