Weems Y S, Bridges P J, Tanaka Y, Sasser R G, LeaMaster B R, Vincent D L, Weems C W
Department of Animal Sciences, University of Hawaii, Honolulu 96822, USA.
Prostaglandins. 1997 May;53(5):337-53. doi: 10.1016/0090-6980(97)00037-3.
Secretion of progesterone in vitro by mature day 8 ovine corpora lutea (CL) of the estrous cycle was increased linearly by ovine LH (1, 10 and 100 ng/ml) or prostaglandin E2 (PGE2) 1, 10 and 100 ng/ml) in a dose dependent manner (P < or = 0.05). Progesterone secretion in vitro by 88-90 day ovine CL of pregnancy was not affected P > or = 0.05 by LH (1, 10 and 100 ng/ml) while prostaglandin E1 (PGE1) 1, 10 and 100 ng/ml) increased (P < or = 0.05) secretion of progesterone in a dose dependent manner and PGE2 (1, 10 and 100 ng/ml) increased (P < or = 0.05) secretion of progesterone only at the 100 ng/ml dose. Day 8 ovine CL of the estrous cycle did not secrete (P > or = 0.05) detectable quantities of prostaglandin F2 alpha (PGF2 alpha) or prostaglandin E (PGE) while 88-90 day ovine CL of pregnancy secrete PGE (P < or = 0.05) but not PGF2 alpha (P > or = 0.05). Regulation of PGE secretion by 88-90 day ovine CL of pregnancy may be via pregnancy specific protein B (PSPB), which increased (P < or = 0.05) PGE and progesterone but not PGF2 alpha (P > or = 0.05) secretion. Secretion of progesterone by CL of 88-90 days of pregnancy was not affected by IGF1, IGF2, PAF-16, PAF-18, oxytocin, PGI2, PGD2 or leukotriene C4 (P > or = 0.05). It is concluded that PGE1 or PGE2 but not LH regulates secretion of progesterone in vitro by 88-90 day ovine CL of pregnancy. In addition, it is concluded that 88-90 day ovine CL of pregnancy secretes it's own luteotropin, which is PGE. Secretion of PGE by ovine CL of pregnancy may be regulated by PSPB.
在发情周期第8天的成熟绵羊黄体（CL）中，体外孕酮分泌量随绵羊促黄体生成素（LH，1、10和100 ng/ml）或前列腺素E2（PGE2，1、10和100 ng/ml）呈剂量依赖性线性增加（P≤0.05）。妊娠88 - 90天的绵羊CL体外孕酮分泌不受LH（1、10和100 ng/ml）影响（P≥0.05），而前列腺素E1（PGE1，1、10和100 ng/ml）呈剂量依赖性增加孕酮分泌（P≤0.05），PGE2（1、10和100 ng/ml）仅在100 ng/ml剂量时增加孕酮分泌（P≤0.05）。发情周期第8天的绵羊CL不分泌（P≥0.05）可检测量的前列腺素F2α（PGF2α）或前列腺素E（PGE），而妊娠88 - 90天的绵羊CL分泌PGE（P≤0.05）但不分泌PGF2α（P≥0.05）。妊娠88 - 90天的绵羊CL对PGE分泌的调节可能通过妊娠特异性蛋白B（PSPB），PSPB增加（P≤0.05）PGE和孕酮分泌，但不增加PGF2α分泌（P≥0.05）。妊娠88 - 90天CL的孕酮分泌不受胰岛素样生长因子1（IGF1）、胰岛素样生长因子2（IGF2）血小板激活因子 - 16（PAF - 16）、血小板激活因子 - 18（PAF - 18）、催产素、前列环素（PGI2）、前列腺素D2（PGD2）或白三烯C4影响（P≥0.05）。结论是，PGE1或PGE2而非LH调节妊娠88 - 90天绵羊CL的体外孕酮分泌。此外，结论是妊娠88 - 90天的绵羊CL分泌自身的促黄体素，即PGE。妊娠绵羊CL的PGE分泌可能受PSPB调节。
