Arkaravichien W, Kendle K E
School of Pharmacy, Robert Gordon's Institute of Technology, Aberdeen, UK.
J Reprod Fertil. 1990 Sep;90(1):63-70. doi: 10.1530/jrf.0.0900063.
The minimum progesterone concentration required to maintain the pregnancy was studied by varying doses of progesterone given subcutaneously to rats ovariectomized on Day 8 of pregnancy. Injecting 3 mg progesterone plus 200 ng oestradiol benzoate daily provided serum progesterone values between 25.4 +/- 7.0 and 35.2 +/- 6.2 ng/ml throughout Days 10-19 which were significantly lower than normal levels (P less than 0.05), but resulted in 93.6% of fetal survival on Day 19 which was not significantly different from 93.3% in the control group. Injecting 2 mg progesterone plus 200 ng oestradiol benzoate daily gave progesterone values between 13.2 +/- 4.6 and 19.0 +/- 6.2 ng/ml and could not maintain fetal viability to Day 19 (14.2%, P less than 0.05 compared with control group). Critical times to supplement progesterone in rats ovariectomized on Day 8 or Day 15 were studied by varying the time of progesterone implantation after ovariectomy. Progesterone implants were administered 8, 12 and 24 h after ovariectomy on Day 8 and 24, 36 and 48 h after ovariectomy on Day 15. On Day 8, progesterone replacement could be delayed to 8 h but not 12 h, while on Day 15, progesterone replacement could be delayed up to 36 h but not 48 h after ovariectomy without affecting fetal survival.
通过对妊娠第8天切除卵巢的大鼠皮下注射不同剂量的孕酮，研究维持妊娠所需的最低孕酮浓度。每天注射3毫克孕酮加200纳克苯甲酸雌二醇，在第10至19天期间，血清孕酮值在25.4±7.0至35.2±6.2纳克/毫升之间，显著低于正常水平（P<0.05），但在第19天胎儿存活率为93.6%，与对照组的93.3%无显著差异。每天注射2毫克孕酮加200纳克苯甲酸雌二醇，孕酮值在13.2±4.6至19.0±6.2纳克/毫升之间，无法维持胎儿存活至第19天（14.2%，与对照组相比P<0.05）。通过改变卵巢切除术后孕酮植入的时间，研究了在妊娠第8天或第15天切除卵巢的大鼠补充孕酮的关键时间。在第8天卵巢切除术后8、12和24小时以及第15天卵巢切除术后24、36和48小时给予孕酮植入物。在第8天，孕酮替代可延迟至8小时，但不能延迟至12小时；而在第15天，卵巢切除术后孕酮替代可延迟至36小时，但不能延迟至48小时，且不影响胎儿存活。
