Al Riyami Bashair, El-Tahir Marah, Al Maskari Sultan, Johnson Eugene H, Saleh Jumana
Department of Biochemistry, College of Medicine, Sultan Qaboos University, Al-Khod, 123 Muscat, Oman.
Department of Microbiology and Immunology, College of Medicine, Sultan Qaboos University, Al-Khod, 123 Muscat, Oman.
J Nutr Metab. 2014;2014:510916. doi: 10.1155/2014/510916. Epub 2014 Dec 2.
Background. ASP, a potent lipogenic factor, was linked to female fat metabolism in association studies. Aim. To investigate acute effects of sex hormone treatment on postprandial ASP levels in vivo. Methods. 24 female rats were randomly divided into 4 groups including controls. The rats were ovariectomized and injected with progesterone, estrogen, or testosterone. An hour later, olive oil was administered orally. Plasma ASP and triglycerides were measured at several postprandial time points. Area under the curve (TG-AUC) represented TG clearance. Results. Only the progesterone treated group had a significant postprandial ASP increase at two hours compared to basal levels (439.8 ± 62.4 versus 253.4 ± 59.03 μg/mL, P = 0.04). Interestingly, increased ASP levels coordinated negatively with corresponding TG levels and TG-AUC postprandially, mostly evident in the opposite effects in the progesterone and testosterone treated groups. ASP levels increased 3-fold in the progesterone versus testosterone treated groups, whereas TG-AUC was significantly lower. Conclusion. These findings suggest that progesterone enhances ASP production and TG clearance simultaneously, supporting the notion of a stimulatory role for progesterone on ASP-mediated TG clearance. This is the first functional study demonstrating a cause-effect relationship between hormone treatment and ASP levels in vivo and may contribute to understanding the mechanism of progesterone function as a female lipogenic hormone.
背景。ASP是一种强效的脂肪生成因子，在关联研究中与女性脂肪代谢相关。目的。研究性激素治疗对体内餐后ASP水平的急性影响。方法。将24只雌性大鼠随机分为包括对照组在内的4组。对大鼠进行卵巢切除，并注射孕酮、雌激素或睾酮。一小时后，口服橄榄油。在几个餐后时间点测量血浆ASP和甘油三酯。曲线下面积（TG-AUC）代表甘油三酯清除率。结果。与基础水平相比，仅孕酮治疗组在两小时时有显著的餐后ASP升高（439.8±62.4对253.4±59.03μg/mL，P = 0.04）。有趣的是，餐后ASP水平升高与相应的甘油三酯水平和TG-AUC呈负相关，在孕酮和睾酮治疗组的相反效应中最为明显。孕酮治疗组的ASP水平相比睾酮治疗组升高了3倍，而TG-AUC显著更低。结论。这些发现表明，孕酮同时增强ASP的产生和甘油三酯清除率，支持孕酮对ASP介导的甘油三酯清除具有刺激作用的观点。这是第一项证明激素治疗与体内ASP水平之间存在因果关系的功能研究，可能有助于理解孕酮作为女性脂肪生成激素的作用机制。
