重新探讨依赖 CRM1 的逆转录病毒 Gag 蛋白的运输。
CRM1-dependent trafficking of retroviral Gag proteins revisited.
机构信息
New York Medical College, Valhalla, New York, USA.
出版信息
J Virol. 2012 Apr;86(8):4696-700. doi: 10.1128/JVI.07199-11. Epub 2012 Feb 8.
Abstract
We analyzed the nuclear trafficking ability of Gag proteins from six retroviral genera. Contrary to a previous report, human immunodeficiency virus type 1 (HIV-1) Gag showed no propensity to cycle through the nucleus. The only Gag protein that displayed CRM1-dependent nuclear cycling was that of Rous sarcoma virus (RSV). Surprisingly, this cycling could be eliminated without compromising infectivity by replacing the RSV Gag N-terminal matrix (MA) domain with HIV MA.
摘要
我们分析了六种逆转录病毒属的 gag 蛋白的核转运能力。与之前的报告相反，人类免疫缺陷病毒 1 型（HIV-1）的 gag 蛋白没有穿过核的倾向。唯一显示 CRM1 依赖性核循环的 gag 蛋白是 Rous 肉瘤病毒（RSV）的 gag 蛋白。令人惊讶的是，通过用 HIV 的 MA 取代 RSV 的 gag N 端基质（MA）结构域，可以在不损害感染性的情况下消除这种循环。
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