Institute of the North Industrial Ecology Problems of Kola Science Centre RAS, Apatity, Russia.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2012;47(3):398-404. doi: 10.1080/10934529.2012.646102.
A new phenomenon has been identified and studied-the impact of bacteria on the benefication process of non-sulphide ores using circulating water supply-a case study of apatite-nepheline ore. It is shown that bacteria deteriorate the floatability of apatite due to their interaction with active centres of calcium-containing minerals and intense flocculation, resulting in a decrease of the flotation process selectivity thus deteriorating the quality of concentrate. Based on the comparative analysis of primary sequences of 16S rRNA genes, there have been identified dominating bacteria species, recovered from the circulating water used at apatite-nepheline concentrating mills, and their phylogenetic position has been determined. All the bacteria were related to γ-Proteobacteria, including the Acinetobacter species, Pseudomonas alcaliphila, Ps. plecoglossicida, Stenotrophomonas rhizophila. A method of non-sulphide ores flotation has been developed with consideration of the bacterial factor. It consists in use of small concentrations of sodium hypochlorite, which inhibits the development of bacteria in the flotation of apatite-nepheline ores.
已鉴定并研究了一种新现象——利用循环供水对非硫化物矿石进行选矿的细菌影响，以磷灰石-霞石矿石为例。结果表明，细菌通过与含钙矿物的活性中心相互作用和强烈的絮凝聚集，降低了磷灰石的可浮性，从而降低了浮选过程的选择性，导致精矿质量变差。基于 16S rRNA 基因的一级序列比较分析，从磷灰石-霞石选矿厂使用的循环水中回收了优势细菌种类，并确定了它们的系统发育位置。所有细菌都与γ-变形菌门有关，包括不动杆菌属、嗜碱假单胞菌、假单胞菌属、寡养单胞菌属。已开发出一种考虑细菌因素的非硫化物矿石浮选方法，其特点是使用低浓度的次氯酸钠，抑制了在浮选磷灰石-霞石矿石过程中细菌的生长。
