State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
Anal Chem. 2012 Feb 21;84(4):1969-74. doi: 10.1021/ac203003w. Epub 2012 Feb 9.
Optical thin films incorporating NaYF(4):Er,Yb upconverting nanorods and chromoionophore ETH 5418 in hydrophobic polymer matrixes have been developed for the first time to measure pH and metal ions based on the ion-exchange mechanism. The absorption spectra of protonated and unprotonated ETH 5418 overlap the two emission peaks of upconverting material, respectively, which makes the inert nanorods ion-sensitive. Optodes for pH and metal ions (Na(+), K(+), Ca(2+), and Cu(2+)) were investigated and exhibited excellent sensitivity, selectivity, and reproducibility. Because of excitation by the 980 nm laser source, detection in the near-infrared region at 656 nm, and high quantum yield of the nanorods in hydrophobic membrane, the proposed sensors have been successfully used in whole blood measurements with minimized background absorption and sample autofluorescence.
首次研制了将上转换纳米棒 NaYF(4):Er,Yb 和显色剂 ETH 5418 掺入疏水性聚合物基质中的光学薄膜，以便基于离子交换机制测量 pH 值和金属离子。质子化和未质子化 ETH 5418 的吸收光谱分别与上转换材料的两个发射峰重叠，这使得惰性纳米棒具有离子敏感性。研究了用于 pH 值和金属离子（Na(+), K(+), Ca(2+), 和 Cu(2+)）的光密度计，它们表现出优异的灵敏度、选择性和重现性。由于采用了 980nm 激光源激发，在近红外区域 656nm 处检测，以及纳米棒在疏水性膜中具有高量子产率，因此所提出的传感器已成功用于全血测量，最小化了背景吸收和样品自荧光。
