Department of Biosciencies, Federal University of Sao Paulo, Santos, Brazil.
Toxicol Mech Methods. 2012 Apr;22(3):218-24. doi: 10.3109/15376516.2011.631196.
The goal of this study was to investigate whether subchronic treatment with grape juice concentrate is able to protect the liver from high fat diet injury in rats. The effects of grape juice concentrate treatment on histopathological changes, and immunohistochemistry for p53, p16 and p21 were evaluated.
Male Wistar rats (n = 18) were distributed into three groups: group 1: negative control; group 2: cholesterol at 1% (w/w) in their diet, treated during 5 weeks; and group 3: cholesterol at 1% in their chow during 5 weeks, and grape juice concentrate at 222 mg per day in their drinking-water in the last week only.
The results pointed out that treatment with grape juice concentrate did not show remarkable differences regarding liver tissue in the cholesterol-exposed group when compared to group 2. However, grape juice concentrate was able to modulate p16 immunoexpression when compared to high fat diet group. p53 and p21 did not show any significant statistical differences among groups.
Taken together, our results suggest that subchronic grape juice concentrate administration was able to modulate cell cycle control by downregulation of p16 immunoexpression in high fat diet-induced liver steatosis in rats.
本研究旨在探讨葡萄汁浓缩物的亚慢性治疗是否能够保护大鼠的肝脏免受高脂肪饮食损伤。评估了葡萄汁浓缩物处理对组织病理学变化以及 p53、p16 和 p21 的免疫组织化学的影响。
将雄性 Wistar 大鼠（n=18）分为三组：第 1 组：阴性对照；第 2 组：饮食中含 1%（w/w）胆固醇，治疗 5 周；第 3 组：饮食中含 1%胆固醇，最后一周仅在饮用水中添加 222mg 葡萄汁浓缩物。
结果表明，与胆固醇暴露组相比，葡萄汁浓缩物治疗组的肝脏组织没有显著差异。然而，与高脂肪饮食组相比，葡萄汁浓缩物能够调节 p16 的免疫表达。p53 和 p21 在各组之间没有显示出任何显著的统计学差异。
综上所述，我们的结果表明，亚慢性葡萄汁浓缩物给药能够通过下调高脂肪饮食诱导的大鼠肝脂肪变性中 p16 的免疫表达来调节细胞周期控制。
