Department of Pathology, Federal University of Sao Paulo, UNIFESP, São Paulo, Brazil.
Environ Sci Pollut Res Int. 2014 Nov;21(22):13118-26. doi: 10.1007/s11356-014-3257-1. Epub 2014 Jul 6.
The aim of this study was to evaluate the antimutagenic and antigenotoxic potential of grape juice concentrate in rodent organs exposed to cadmium chloride intoxication. A total of 15 Wistar rats were distributed into three groups (n = 5), as follows: control group (CTRL; nontreated group), cadmium group (Cd), and cadmium-grape juice group (Cd + GJ). Exposed animals received intraperitoneal injection of cadmium chloride (1.2 mg/kg body weight) diluted in water and, after 15 days, Cd + GJ group received grape juice concentrate for 15 days, by gavage (0.8 mL, 1.18 mg of polyphenols kg(-1) day(-1)). Grape juice concentrate was able to decrease genotoxic effects induced by cadmium in peripheral blood and liver cells as depicted by single cell gel (comet) and micronucleus assays. A decrease for anti-8-hydroxy-20-deoxyguanosine (8OHdG) expression in hepatocytes of animals exposed to cadmium and treated with grape juice concentrate was also detected. Higher CuZn-SOD activity was observed in liver cells of the Cd + GJ group. No remarkable differences were seen regarding Mn-SOD activity among groups. Taken together, our results demonstrate that grape juice concentrate was able to exert antimutagenic and antigenotoxic activities in blood and liver cells of rats exposed to cadmium.
本研究旨在评估浓缩葡萄汁在氯化镉中毒的啮齿动物器官中的抗突变和抗原毒潜力。将 15 只 Wistar 大鼠随机分为三组（n = 5）：对照组（CTRL；未处理组）、镉组（Cd）和镉-葡萄汁组（Cd + GJ）。暴露于镉的动物接受腹腔注射氯化镉（1.2 mg/kg 体重）稀释在水中，15 天后，Cd + GJ 组通过灌胃接受浓缩葡萄汁 15 天（0.8 mL，1.18 mg 多酚/kg(-1)天(-1))。浓缩葡萄汁能够降低外周血和肝细胞中由镉引起的遗传毒性效应，如单细胞凝胶（彗星）和微核试验所示。还检测到暴露于镉并经葡萄汁浓缩物处理的动物肝细胞中抗 8-羟基-20-脱氧鸟苷（8OHdG）表达减少。Cd + GJ 组肝细胞中 CuZn-SOD 活性较高。各组间 Mn-SOD 活性无明显差异。总之，我们的结果表明，浓缩葡萄汁能够对暴露于镉的大鼠血液和肝细胞发挥抗突变和抗原毒作用。
