周期性 48 小时禁食可通过促进脂肪生成和脂生成来改善生长猪的葡萄糖耐量。

Periodic 48 h feed withdrawal improves glucose tolerance in growing pigs by enhancing adipogenesis and lipogenesis.

机构信息

Agriculture and Agri-food Canada Research Centre, 5403, 1st Ave S,, P,O, Box 3000, Lethbridge, AB T1J 4B1, Canada.

出版信息

Nutr Metab (Lond). 2012 Feb 9;9(1):10. doi: 10.1186/1743-7075-9-10.

DOI:10.1186/1743-7075-9-10

PMID:22321818

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3292934/

Abstract

BACKGROUND

Adipocyte numbers and peroxisome proliferators activated receptorγ (PPARγ) expression of retroperitoneal tissue increased while area under the curve (AUC) during the glucose tolerance test (GTT) was reduced in rats subjected to certain feed withdrawal (FW) regimens. Thus, using pigs as the experimental model, the hypothesis that FW regimens influence glucose tolerance by influencing fat cell function was evaluated with the objective of determining the effect of a single (FWx1; at age of 19 wk for 48 h) or periodic, multiple (FWx4; 24 h FW at 7 and 11 wk of age and 48 h FW at 15 and 19 wk of age) FW on AUC of glucose and insulin during the GTT relative to pigs that did not experience FW (Control).

METHODS

Growth, body composition, adipocyte numbers, PPARγ expression, lipogenic potential as glucose uptake into fat of adipocytes of varying diameter in omental (OM) and subcutaneous (SQ) fat as affected by FW regimens were determined in pigs initiated into the study at 5 wk of age and fed the same diet, ad libitum.

RESULTS

Blood glucose concentrations for prior to and 120 min post glucose meal tended to be lower (p = 0.105 and 0.097, respectively) in pigs in FW treatments. In OM fat; cell numbers, glucose Universal14C [U14C] incorporation into fat and rate of incorporation per 104 cells was greatest for cells with diameters of 90-119 μm. Pigs undergoing FWx4 tended to have greater (p = 0.0685; by 191%) number of adipocytes, increased (p = 0.0234) glucose U14C incorporation into adipocytes and greater (p = 0.0872) rate of glucose uptake into cells of 119-150 μm diameter than of cells from control or FWx1 pigs. Subcutaneous adipocyte numbers in 22-60 and 61-90 μm diameter ranges from pigs in FWx1 tended to be greater (p = 0.08 and 0.06, respectively) than for those in FWx4 treatment, yet PPARγ expression and total cell number were not affected by treatment.

CONCLUSIONS

Results suggest that FW regimens influence fat cell function or lipogenesis rather than number, affecting glucose metabolism and may have implications in drug-free control of metabolic syndrome symptoms.

摘要

背景

在接受某些禁食（FW）方案的大鼠中，腹膜后组织的脂肪细胞数量和过氧化物酶体增殖物激活受体γ（PPARγ）表达增加，而葡萄糖耐量试验（GTT）的曲线下面积（AUC）减少。因此，本研究以猪为实验模型，通过评估 FW 方案对脂肪细胞功能的影响是否会影响葡萄糖耐量，提出了 FW 方案通过影响脂肪细胞功能影响葡萄糖耐量的假设，目的是确定单次（FWx1；19 周龄时禁食 48 小时）或周期性多次（FWx4；7 周和 11 周龄时禁食 24 小时，15 周和 19 周龄时禁食 48 小时）FW 对 GTT 期间葡萄糖和胰岛素 AUC 的影响，与未经历 FW（对照）的猪相比。

方法

在 5 周龄时开始进行研究并自由喂食相同饮食的猪中，确定了 FW 方案对生长、体成分、脂肪细胞数量、PPARγ 表达、不同直径的网膜（OM）和皮下（SQ）脂肪中的脂肪细胞的生脂潜力（作为葡萄糖摄取到脂肪中的能力）的影响。

结果

在 FW 处理组中，葡萄糖餐之前和 120 分钟后的血糖浓度趋于较低（p=0.105 和 0.097）。在 OM 脂肪中，直径为 90-119μm 的细胞的细胞数量、葡萄糖通用 14C[U14C]掺入脂肪和每 104 个细胞的掺入率最高。进行 FWx4 的猪的脂肪细胞数量有增加的趋势（p=0.0685；增加 191%），葡萄糖 U14C 掺入脂肪细胞的量增加（p=0.0234），直径为 119-150μm 的细胞的葡萄糖摄取率增加（p=0.0872），而直径为 22-60μm 和 61-90μm 范围内的细胞的葡萄糖摄取率低于对照组或 FWx1 猪。FWx1 猪的 22-60μm 和 61-90μm 直径范围的皮下脂肪细胞数量趋于较高（p=0.08 和 0.06），而 FWx4 处理的脂肪细胞数量则较高，但处理对 PPARγ 表达和总细胞数没有影响。