Teng Jian-Ying, Guo Rui, Xie Jing, Sun Dong-Jie, Wu Jia-Jia, Pang Xin-Nian, Shen Ming-Qiang, Xu Shao-Jun
Clinical Medical College, Hangzhou Normal University, Hangzhou 310036, China.
Zhonghua Yi Xue Za Zhi. 2011 Sep 27;91(36):2568-72.
To investigate the effects of vascular endothelial growth factor (VEGF) DNA plasmids, encoded in collagen-sulfonated carboxymethyl chitosan porous scaffold, on the angiogenesis in full-thickness burn wounds.
Wounds and pathological changes were observed at Week 1, 2, 3 and 4 after pure collagen-sulfonated carboxymethyl chitosan porous scaffold (group A) and collagen-sulfonated carboxymethyl chitosan porous scaffold encoding VEGF DNA plasmids (group B) were transplanted onto eachar-removed wounds of full-thickness burn in 6 Bama miniature pigs respectively. Wound healing was observed by pathologic slides after epidermal grafting for 2 weeks (at Week 4) onto the surface of different dermal scaffolds transplanted on wounds for 2 weeks. At the same time, new-forming vessels expressing CD31 and mature vessels expressing α-SMA (α-smooth muscle actin) and the expression of VEGF in wounds at Week 1, 2, 3 and 4 were detected in situ by immunohistochemical staining. The burn wounds without any transplanted scaffold (group C) were studied as the controls.
Wounds with various scaffolds were different from those without any scaffold. Angiogenesis of the group B was better than that of the group A. Neo-forming micro-vessels expressing CD31 in the wounds of group A or B at Week 1, 2, 3 and 4 were as follows: 18.7 ± 3.1, 25.7 ± 2.3, 36.8 ± 2.5 & 26.2 ± 2.9; 24.5 ± 3.8, 32.3 ± 2.8, 39.2 ± 2.2 & 27.3 ± 3.0. Mature vessels expressing α-SMA: 11.7 ± 1.9, 20.5 ± 1.9, 35.0 ± 4.5 & 24.0 ± 2.8; 20.2 ± 3.1, 33.5 ± 3.7, 38.2 ± 4.5 & 26.5 ± 2.3. The expressions of VEGF: 48.7 ± 7.9, 141.7 ± 9.1, 201.5 ± 8.6 & 107.0 ± 8.2; 97.3 ± 7.9, 172.3 ± 8.1, 208.7 ± 8.3 & 114.0 ± 5.8. Expressing intensity of CD31, α-SMA and VEGF in the wounds of group C at Week 1, 2, 3 & 4: 6.0 ± 2.0, 9.8 ± 3.4, 19.3 ± 2.5 & 18.7 ± 2.2; 3.7 ± 2.0, 8.7 ± 1.8, 13.0 ± 2.5 & 14.0 ± 2.8; 3.5 ± 2.3, 10.3 ± 3.5, 23.0 ± 5.6 & 21.5 ± 5.1. There were significant statistical differences among three groups.
Artificial dermal scaffold encoding exogenous pDNA-VEGF can promote a quicker angiogenesis through a high expression of VEGF. Thus a full-thickness burn wound may be better repaired.
研究胶原 - 磺化羧甲基壳聚糖多孔支架中编码的血管内皮生长因子（VEGF）DNA质粒对全层烧伤创面血管生成的影响。
将纯胶原 - 磺化羧甲基壳聚糖多孔支架（A组）和编码VEGF DNA质粒的胶原 - 磺化羧甲基壳聚糖多孔支架（B组）分别移植到6只巴马小型猪的全层烧伤去皮创面上，于术后第1、2、3和4周观察创面及病理变化。在移植到创面上2周的不同真皮支架表面进行表皮移植2周（第4周）后，通过病理切片观察创面愈合情况。同时，采用免疫组织化学染色原位检测第1、2、3和4周创面中表达CD31的新生血管、表达α - SMA（α - 平滑肌肌动蛋白）的成熟血管以及VEGF的表达。以未移植任何支架的烧伤创面（C组）作为对照。
移植不同支架的创面与未移植支架的创面不同。B组的血管生成情况优于A组。A组或B组创面在第1、2、3和4周表达CD31的新生微血管数量分别为：18.7±3.1、25.7±2.3、36.8±2.5和26.2±2.9；24.5±3.8、32.3±2.8、39.2±2.2和27.3±3.0。表达α - SMA的成熟血管数量分别为：11.7±1.9、20.5±1.9、35.0±4.5和24.0±2.8；20.2±3.1、33.5±3.7、38.2±4.5和26.5±2.3。VEGF的表达量分别为：48.7±7.9、141.7±9.1、201.5±8.6和107.0±8.2；97.3±7.9、172.3±8.1、208.7±8.3和114.0±5.8。C组创面在第1、2、3和4周CD31、α - SMA和VEGF的表达强度分别为：6.0±2.0、9.8±3.4、19.3±2.5和18.7±2.2；3.7±2.0、8.7±1.8、13.0±2.5和14.0±2.8；3.5±2.3、10.3±3.5、23.0±5.6和21.5±5.1。三组之间存在显著统计学差异。
编码外源性pDNA - VEGF的人工真皮支架可通过VEGF的高表达促进更快的血管生成，从而更好地修复全层烧伤创面。
