Laboratory of Experimental Pneumology, IDIBELL, L'Hospitalet, Barcelona, Spain.
Respir Physiol Neurobiol. 2012 Apr 15;181(1):46-52. doi: 10.1016/j.resp.2012.01.012. Epub 2012 Feb 2.
Bleomycin injury causes biomechanical changes secondary to inflammation, tissue remodeling and surfactant changes. We compared lung mechanics in open chest (OC) and tissue strip (TS) to better understand the pathophysiology of the alveolar interface between lung tissue and conducting airways. Thirty nine rats were studied at days 3, 7, and 15 after receiving saline or bleomycin (2.5 Ukg(-1)) intratracheally. Normalized elastance (E), hysteresivity (η) and exponent (β) of the power frequency dependence of elastance were determined in OC (lung parenchyma) and TS. Remodeling (hydroxyproline) and inflammation (myeloperoxidase and lung water) parameters were determined. E, η and β were higher in OC both in saline and bleomycin groups. The difference (OC-TS) of η and β correlated with myeloperoxidase and lung water but not with hydroxyproline. We concluded that differences between lung parenchyma and tissue mechanics are due to mechanical effects of inhomogeneities in saline animals. Changes at the alveolar interface after bleomycin are related to oxidative stress and extravascular lung water.
博莱霉素损伤导致炎症、组织重塑和表面活性剂变化引起的生物力学变化。我们比较了开胸（OC）和组织条（TS）的肺力学，以更好地理解肺泡界面的病理生理学，即肺组织与传导气道之间的界面。在接受生理盐水或博莱霉素（2.5 U/kg）气管内给药后第 3、7 和 15 天，对 39 只大鼠进行了研究。在 OC（肺实质）和 TS 中确定了标准化弹性（E）、滞后性（η）和弹性的功率频率依赖性指数（β）。测定了重塑（羟脯氨酸）和炎症（髓过氧化物酶和肺水）参数。OC 中 E、η 和 β 在生理盐水和博莱霉素组中均较高。η 和 β 的 OC-TS 差异与髓过氧化物酶和肺水相关，但与羟脯氨酸无关。我们得出结论，肺实质和组织力学之间的差异是由于盐水动物中不均匀性的力学效应所致。博莱霉素后肺泡界面的变化与氧化应激和血管外肺水有关。
