Lindström Sara
Department of Cell and Molecular Biology, Science For Life Laboratory, Karolinska Institute, Stockholm, Sweden.
Methods Mol Biol. 2012;853:13-5. doi: 10.1007/978-1-61779-567-1_2.
Flow cytometry and microscopy are perhaps the two most commonly used techniques for analyzing single cells. Both techniques are typically robust and provide a high throughput analysis of living and/or fixed cells. The techniques are often combined with fluorescent labeling of cells, using for example antibodies. This chapter is a short introductory review where some of the possible applications using flow cytometry and microscopy are discussed.
流式细胞术和显微镜术或许是分析单个细胞最常用的两种技术。这两种技术通常都很可靠,能对活细胞和/或固定细胞进行高通量分析。这些技术常与细胞的荧光标记相结合,例如使用抗体。本章是一篇简短的介绍性综述,讨论了一些使用流式细胞术和显微镜术的可能应用。