Cho H Y, Rhim J S
Science. 1979 Aug 17;205(4407):691-3. doi: 10.1126/science.223242.
The protein synthesis inhibitor cycloheximide, at a concentration of 0.08 microgram per milliliter, induced flat morphology within 24 to 48 hours and low saturation density in human osteosarcoma cells transformed by Kirsten murine sarcoma virus (Ki-MSV) or N-methyl-N' nitro-N-nitrosoguanidine. Removal of the protein synthesis inhibitor caused both transformed cells to revert to the transformed phenotype. The demonstration of cell-surface antigens, cross-reacted with antiserums induced by extracts of both types of transformed human cells, was dependent on the presence or absence of cycloheximide in the culture medium. The results show that protein synthesis is required to maintain the transformed state in virally or chemically transformed human cells.
蛋白质合成抑制剂放线菌酮，浓度为每毫升0.08微克，在24至48小时内可诱导由 Kirsten 小鼠肉瘤病毒（Ki-MSV）或N-甲基-N'-硝基-N-亚硝基胍转化的人骨肉瘤细胞呈现扁平形态和低饱和密度。去除蛋白质合成抑制剂会使两种转化细胞都恢复到转化表型。与两种类型的转化人细胞提取物诱导的抗血清发生交叉反应的细胞表面抗原的展示，取决于培养基中放线菌酮的存在与否。结果表明，蛋白质合成是维持病毒或化学转化的人细胞的转化状态所必需的。
