Rhim J S, Putman D L, Arnstein P, Huebner R J, McAllister R M
Int J Cancer. 1977 Apr 15;19(4):505-10. doi: 10.1002/ijc.2910190411.
Human osteosarcoma (HOS) clonal cells transformed in vitro by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) were characterized, and compared to non-producer HOS cells transformed by Kirsten murine sarcoma virus (Ki-MSV). The MNNG- and virus-transformed cells grew in the aggregate form above an agar base, grew in soft agar, and had a high fibrinolytic activity. When inoculated into nude mice, all the chemically or virally altered cells produced tumors or tumor nodules. When transplanted into ATS-treated hamsters, the cells transformed by MNNG (0.01 mug/ml) and Ki-MSV produced tumors but MNNG (0.1 mug/ml) transformed cells did not produce tumors. The control HOS cells did not grow in the aggregate form but formed colonies in soft agar, and had low fibrinolytic activity and no capacity to form tumors in nude mice and ATS-treated hamsters. However, one of the control clonal lines had a high level of fibrinolytic activity. Cellular aggregation properties of human transformed cells did appear to correlate with tumorigenicity in nude mice.
对经N-甲基-N'-硝基-N-亚硝基胍（MNNG）体外转化的人骨肉瘤（HOS）克隆细胞进行了表征，并与经 Kirsten 鼠肉瘤病毒（Ki-MSV）转化的非产生型HOS细胞进行了比较。MNNG和病毒转化的细胞在琼脂基质上以上皮细胞聚集的形式生长，能在软琼脂中生长，并且具有高纤溶活性。当接种到裸鼠体内时，所有化学或病毒改变的细胞都会产生肿瘤或肿瘤结节。当移植到经ATS处理的仓鼠体内时，经MNNG（0.01微克/毫升）和Ki-MSV转化的细胞会产生肿瘤，但经MNNG（0.1微克/毫升）转化的细胞不会产生肿瘤。对照HOS细胞不会以上皮细胞聚集的形式生长，但能在软琼脂中形成集落，具有低纤溶活性，并且在裸鼠和经ATS处理的仓鼠中没有形成肿瘤的能力。然而，其中一个对照克隆系具有高水平的纤溶活性。人转化细胞的细胞聚集特性似乎与裸鼠中的致瘤性相关。
