马鹿（Cervus elaphus L.）β-乳球蛋白制剂在接近中性pH值时的聚集状态。
The state of aggregation of red deer (Cervus elaphus L.) beta-lactoglobulin preparations near neutral pH.
作者信息
McDougall E I, Stewart J C
出版信息
Biochem J. 1977 Oct 1;167(1):45-51. doi: 10.1042/bj1670045.
Abstract
- The state of aggregation of four red-deer (Cervus elaphus L.) beta-lactoglobulin preparations and a control ox beta-lactoglobulin A preparation was studied by sedimentation-equilibrium experiments at pH 6.5 and 20 degrees C. 2. Three of the deer preparations and the ox control each behaved as a monomer-dimer system, with a value of log K (where K is the association constant in litres/mol) in the range 5.4-5.5. 3. When one of these deer preparations was examined in the presence of dithiothreitol, log K appeared to decrease to 4.5.4. One deer preparation, comprising recovered material, appeared to have undergone irreversible changes and to behave like a non-equilibrating system containing monomer, dimer and trimer. 5. The sedimentation-equilibrium properties of the deer monomer was studied in 6M-guanidine hydrochloride at pH 7.0; the mol.wt. was 17600, the second virial coefficient was 3.4 x 10(-3) ml - mol - g-2, and the apparent partial specific volume 0.724 ml/g, a value indicating an appreciable decrease in volume on dissociation and denaturation.
摘要
- 通过在pH 6.5和20摄氏度下进行沉降平衡实验，研究了四种马鹿（Cervus elaphus L.）β-乳球蛋白制剂和一种对照牛β-乳球蛋白A制剂的聚集状态。2. 三种鹿制剂和牛对照制剂均表现为单体-二聚体系统，log K值（其中K是升/摩尔的缔合常数）在5.4 - 5.5范围内。3. 当在二硫苏糖醇存在下检查其中一种鹿制剂时，log K似乎降至4.5。4. 一种由回收材料组成的鹿制剂似乎发生了不可逆变化，表现得像一个包含单体、二聚体和三聚体的非平衡系统。5. 在pH 7.0的6M盐酸胍中研究了鹿单体的沉降平衡性质；分子量为17600，第二维里系数为3.4×10⁻³毫升·摩尔·克⁻²，表观比容为0.724毫升/克，该值表明解离和变性时体积有明显减小。
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