鹅细小病毒前体 mRNA 的剪接影响加工 mRNA 的细胞质翻译。
Splicing of goose parvovirus pre-mRNA influences cytoplasmic translation of the processed mRNA.
机构信息
Department of Molecular Microbiology and Immunology, University of Missouri-Columbia School of Medicine, Bond Life Sciences Center, Columbia, MO, USA.
出版信息
Virology. 2012 Apr 25;426(1):60-5. doi: 10.1016/j.virol.2012.01.019. Epub 2012 Feb 10.
Translation of goose parvovirus (GPV) 72 kDa Rep 1 is initiated from unspliced P9-generated mRNAs in ORF1 from the first in-frame AUG (537 AUG); however, this AUG is bypassed in spliced P9-generated RNA: translation of the 52 kDa Rep 2 protein from spliced RNA is initiated in ORF2 at the next AUG downstream (650 AUG). Usage of the 537 AUG was restored in spliced RNA when the GPV intron was replaced with a chimeric SV40 intron, or following specific mutations of the GPV intron which did not appear in the final spliced mRNA. Additionally, 650 AUG usage was gained in unspliced RNA when the GPV intron splice sites were debilitated. Splicing-dependent regulation of translation initiation was mediated in cis by GPV RNA surrounding the target AUGs. Thus, nuclear RNA processing of GPV P9-generated pre-mRNAs has a complex, but significant, effect on alternative translation initiation of the GPV Rep proteins.
鹅细小病毒（GPV）72 kDa Rep 1 的翻译起始于 ORF1 中未剪接 P9 生成的 mRNA 中的第一个框内 AUG（537 AUG）；然而，该 AUG 在剪接 P9 生成的 RNA 中被绕过：从拼接 RNA 中的 ORF2 起始翻译 52 kDa Rep 2 蛋白，位于下游的下一个 AUG（650 AUG）。当 GPV 内含子被嵌合 SV40 内含子取代时，或者当 GPV 内含子发生特定突变但最终拼接的 mRNA 中未出现这些突变时，拼接 RNA 中 537 AUG 的使用得到恢复。此外，当 GPV 内含子剪接位点失活时，未剪接 RNA 中获得了 650 AUG 的使用。翻译起始的剪接依赖性调节是由目标 AUG 周围的 GPV RNA 顺式介导的。因此，GPV P9 生成的前体 mRNA 的核 RNA 加工对 GPV Rep 蛋白的替代翻译起始有复杂但重要的影响。
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