College of Environmental Science and Engineering, South China University of Technology, Guangzhou, Guangdong 510006, PR China.
Bioresour Technol. 2012 Apr;110:297-301. doi: 10.1016/j.biortech.2011.12.120. Epub 2012 Jan 3.
Modification of the physical properties of old corrugated container (OCC) pulp with laccase or a laccase-mediator (ABTS, HBT, VA) system was investigated under select enzymatic concentrations and reaction times. The optimal conditions for laccase treatment shown to be using a laccase dose of 160U/g o.d. pulp, a treatment time of 20h at 25°C, pH 7 with a pulp consistency of 5%. Results showed that the Lac-HBT treated OCC pulp gave the best strength properties, improving tensile strength by 15.7%. The increase in the carboxyl group content of OCC laccase or Lac-HBT treated pulp led to the increase in the swelling ability and bonding between fibers. Microscope images showed the fiber surface became rougher and more collapsible after Lac-HBT treatment. FT-IR data showed that new carboxylic acid groups were formed during Lac-HBT treatment.
用漆酶或漆酶-介体（ABTS、HBT、VA）体系对旧瓦楞纸箱（OCC）纸浆的物理性能进行改性，在选择的酶浓度和反应时间下进行了研究。结果表明，漆酶处理的最佳条件为：用酶量 160U/g 绝干浆，在 25°C、pH7、浆浓 5%下处理 20h。结果表明，Lac-HBT 处理的 OCC 浆粕的强度性能最佳，拉伸强度提高了 15.7%。OCC 漆酶或 Lac-HBT 处理的纸浆中羧基含量的增加导致了纤维润胀能力和纤维间结合力的提高。显微镜图像显示，Lac-HBT 处理后纤维表面变得更粗糙，更易坍塌。FT-IR 数据表明，在 Lac-HBT 处理过程中形成了新的羧酸基团。
