UR1268 Biopolymères Interactions Assemblages, INRA, F-44300 Nantes, France.
Colloids Surf B Biointerfaces. 2012 Jun 1;94:369-73. doi: 10.1016/j.colsurfb.2012.01.024. Epub 2012 Jan 27.
This communication describes the fabrication of microstructured biopolymer surfaces by the site-selective capture of pectin hydrogel beads. A positively charged surface consisting of poly-L-lysine (PLL) was subjected to site-selective enzymatic degradation using patterned polydimethylsiloxane (PDMS) stamps covalently modified with trypsin, according to the recently described method. The patterned surface was used to capture ionically cross-linked pectin beads. The desired patterning of the hydrogel surfaces was generated by site-selective immobilization of these pectin beads. The ability of the hydrogels to be dried and swollen in water was assessed.
本通讯描述了通过果胶水凝胶珠的选择性捕获来制造微结构的生物聚合物表面。根据最近描述的方法，使用共价修饰有胰蛋白酶的图案化聚二甲基硅氧烷（PDMS）印章对带正电荷的包含聚-L-赖氨酸（PLL）的表面进行选择性酶降解。该图案化表面用于捕获离子交联的果胶珠。通过这些果胶珠的选择性固定，生成了所需的水凝胶表面的图案化。评估了水凝胶在干燥和水中溶胀的能力。
