Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330, Etiler Ankara, Turkey.
J Ethnopharmacol. 2012 Mar 27;140(2):409-15. doi: 10.1016/j.jep.2012.01.042. Epub 2012 Feb 2.
Juniperus (Cupressaceae) species are widely used as folk medicine in spreading countries. Decoction of Juniperus oxycedrus subsp. oxycedrus L. leaves is used internally to lower blood glucose levels in Turkey.
To determine hypoglycaemic and antidiabetic activities of Juniperus oxycedrus subsp. oxycedrus leaves and to identify active compounds through bioactivity guided isolation technique.
Ethanol and water extracts of Juniperus oxycedrus subsp. oxycedrus (Joso), leaves on oral administration were studied using in vivo models in normal, glucose-hyperglycemic and streptozotocin-induced diabetic rats. Through in vivo bioactivity-guided fractionation processes, a nonpolar fraction was separated from the n-hexane subextract by silica gel column chromatography as the main active fraction. Subfractions of this fraction was found to possess antidiabetic activity and their chemical composition was investigated by GC-FID and GC-MS, simultaneously.
This is the first report on the antidiabetic constituents of Joso leaves. Fatty acids, such as palmitic, linoleic and linolenic acid were found as the major compounds in subfractions.
Results indicated that Joso leaf extract and its active constituents might be beneficial for diabetes mellitus.
柏科（Cupressaceae）物种在传播国家被广泛用作民间药物。土耳其人将 Juniperus oxycedrus subsp. oxycedrus L. 的叶子煮成汤，用于降低血糖水平。
确定 Juniperus oxycedrus subsp. oxycedrus 叶的降血糖和抗糖尿病活性，并通过基于生物活性的分离技术鉴定活性化合物。
使用正常、葡萄糖高血糖和链脲佐菌素诱导的糖尿病大鼠的体内模型，研究 Juniperus oxycedrus subsp. oxycedrus（Joso）叶的乙醇和水提取物的口服给药。通过体内生物活性导向的分级分离过程，从正己烷亚提取物中通过硅胶柱色谱法分离出非极性级分作为主要活性级分。发现该级分的亚级分具有抗糖尿病活性，并通过 GC-FID 和 GC-MS 同时研究其化学成分。
这是首次报道 Joso 叶的抗糖尿病成分。发现脂肪酸，如棕榈酸、亚油酸和亚麻酸是亚级分中的主要化合物。
结果表明，Joso 叶提取物及其活性成分可能有益于糖尿病。
