Transdisciplinary Science and Engineering Program, Graduate School of Advanced Science and Engineering, Hiroshima University, Hiroshima 739-8529, Japan.
Division of Hematology, Department of Internal Medicine, Aichi Medical University School of Medicine, Nagakute 480-1195, Japan.
Molecules. 2022 Apr 2;27(7):2307. doi: 10.3390/molecules27072307.
This is the first study to examine the effects of in vitro digestion on biological activities of spp., a broadly known brown seaweed for therapeutic potential. Three fractions (F1-F3) were obtained from hexane extract by column chromatography. Under in vitro simulated digestion, the anti-α-amylase capacity of F1 in oral and intestinal phases increases, while it significantly decreases in the gastric phase. The α-amylase inhibition of F2 promotes throughout all digestive stages while the activity of F3 significantly reduces. The cytotoxic activity of F1 against U266 cell-line accelerates over the oral, gastric, and intestinal stages. The fractions F2 and F3 exhibited the declined cytotoxic potentialities in oral and gastric phases, but they were strengthened under intestinal condition. Palmitic acid and fucosterol may play an active role in antidiabetic and cytotoxic activity against multiple myeloma U266 cell line of spp. However, the involvement of other phytochemicals in the seaweed should be further investigated.
这是第一项研究，旨在探讨体外消化对 spp.（一种被广泛认可的具有治疗潜力的褐藻）生物活性的影响。通过柱层析从正己烷提取物中得到三个馏分（F1-F3）。在体外模拟消化过程中，F1 在口腔和肠道阶段的抗α-淀粉酶能力增加，而在胃阶段则显著降低。F2 的α-淀粉酶抑制作用在所有消化阶段均促进，而 F3 的活性则显著降低。F1 对 U266 细胞系的细胞毒性作用在口腔、胃和肠道阶段均加速。F2 和 F3 馏分在口腔和胃阶段的细胞毒性潜力下降，但在肠道条件下增强。棕榈酸和岩藻甾醇可能在抗糖尿病和对多发性骨髓瘤 U266 细胞系的细胞毒性活性中发挥作用。然而，海藻中其他植物化学物质的参与还需要进一步研究。
