Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China.
Exp Parasitol. 2012 Apr;130(4):412-5. doi: 10.1016/j.exppara.2012.01.019. Epub 2012 Feb 4.
Cysteine proteinases 4 (EhCP4) of Entamoeba histolytica are considered important for ameba pathogenicity. The recombinant gene was obtained by cloning and expression of the EhCP4 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in minipig against challenge infection in a minipig-E. histolytica model. There was a 53.16% reduction (P<0.001) in the group of recovery of challenged E. histolytica compared with that in the control group. Specific anti-EhCP4 antibodies from immune protected minipig had significantly higher levels of immunoglobulin G (IgG) (P<0.001). This is a first report demonstrating that a recombinant form of EhCP4 generated in E. coli, to immunize a minipig model of E. histolytica, and there is significant protection. This study may help to understand the EhCP4 for human in the future.
半胱氨酸蛋白酶 4（EhCP4）被认为是溶组织内阿米巴的重要致病因子。通过在异源宿主大肠杆菌 BL-21（DE3）中克隆和表达 EhCP4 基因获得重组基因，用于评估其在猪阿米巴病模型中诱导针对挑战感染的免疫保护反应的能力。与对照组相比，回收的受挑战的溶组织内阿米巴的数量减少了 53.16%（P<0.001）。来自免疫保护的小猪的特异性抗 EhCP4 抗体具有显著更高水平的免疫球蛋白 G（IgG）（P<0.001）。这是首次报道表明，在大肠杆菌中产生的重组形式的 EhCP4 可以免疫猪的溶组织内阿米巴病模型，并具有显著的保护作用。这项研究可能有助于未来了解 EhCP4 对人类的作用。
