Guiyang College of Traditional Chinese Medicine, Guiyang 550002, Guizhou Province, China.
Exp Parasitol. 2012 Mar;130(3):253-6. doi: 10.1016/j.exppara.2011.12.004. Epub 2011 Dec 19.
Entamoeba histolytica cysteine proteinase gene 5(EhCP5) is one of the major proteinase genes of all EhCP-transcripts. The amebiasis cysteine proteinase gene encoding an antigen from E. histolytica, as well as the recombinant EhCP5, obtained by cloning and expression of the EhCP5 gene in heterologous host Escherichia coli BL-21 (DE3), were used to evaluate their ability to induce immune protective responses in Minipig against challenge infection in a minipig-E. histolytica model. There was a 52.27% reduction (P<0.001) in the group of recovery of challenged E. histolytica compared with that in the control group. Specific anti-EhCP5 antibodies from immune protected minipig had significantly higher levels of immunoglobulin G (IgG) (P<0.0001). Our data will help to know the mechanism of vaccinal protection of E. histolytica.
溶组织内阿米巴半胱氨酸蛋白酶基因 5（EhCP5）是所有 EhCP-转录本的主要蛋白酶基因之一。用编码来自溶组织内阿米巴的抗原的阿米巴病半胱氨酸蛋白酶基因，以及通过在异源宿主大肠杆菌 BL-21（DE3）中克隆和表达 EhCP5 基因获得的重组 EhCP5，用于评估它们在针对猪模型中的挑战感染的免疫保护反应中的能力。与对照组相比，从受保护的感染猪中恢复的组中，溶组织内阿米巴的恢复减少了 52.27%（P<0.001）。来自免疫保护猪的特异性抗-EhCP5 抗体具有显著更高水平的免疫球蛋白 G（IgG）（P<0.0001）。我们的数据将有助于了解溶组织内阿米巴疫苗保护的机制。
