National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Appl Biochem Biotechnol. 2012 Apr;166(7):1781-90. doi: 10.1007/s12010-012-9586-y. Epub 2012 Feb 11.
A mutant Cs3512, which showed better fermentation of xylose and the mixtures of xylose and glucose, was obtained through mutation of Candida shehatae ATCC 22984 and screening with a medium containing antimycin A and TTC (2,3,5-triphenyltetrazolium chloride). Cs3512 produced 44.4 g/l of ethanol from 121.3 g/l of xylose, which was 13% higher than that by ATCC 22984. At the same time, xylitol production was reduced by 38% to 10.2 g/l from 16.3 g/l by ATCC 22984. Cs3512 also showed 8% increase in ethanol yield from 0.39 to 0.42 g/g comparing to ATCC 22984 when fermenting the sugar mixture composed of 52.9 g/l glucose and 21.2 g/l xylose. When Cs3512 was used in the simultaneous saccharification and fermentation of lime pretreated rice straw via CaCCO (calcium capturing by carbonation) process, it produced ethanol at 77% of the theoretical yield. The results imply that Cs3512 is a potential non-recombinant yeast strain for ethanol production from lignocellulosic biomass.
通过突变 Candida shehatae ATCC 22984 并筛选含有安密妥和 TTC（2,3,5-三苯基氯化四氮唑）的培养基，获得了突变株 Cs3512，其在木糖和木糖与葡萄糖的混合物发酵方面表现出更好的性能。Cs3512 从 121.3 g/L 的木糖中生产了 44.4 g/L 的乙醇，比 ATCC 22984 提高了 13%。同时，木糖醇的产量从 ATCC 22984 的 16.3 g/L 降低了 38%，至 10.2 g/L。与 ATCC 22984 相比，Cs3512 在发酵由 52.9 g/L 葡萄糖和 21.2 g/L 木糖组成的糖混合物时，乙醇得率从 0.39 提高到 0.42 g/g，提高了 8%。当 Cs3512 用于通过 CaCCO（碳酸化捕获钙）工艺对石灰预处理的水稻秸秆进行同步糖化和发酵时，它产生了 77%的理论乙醇产量。结果表明，Cs3512 是一种具有潜力的非重组酵母菌株，可用于从木质纤维素生物质生产乙醇。
