Ouyang Dong
Armed Police Corps Hospital of Fujian, Fuzhou, China.
Hepatogastroenterology. 2012 Oct;59(119):2314-7. doi: 10.5754/hge11839.
BACKGROUND/AIMS: ¹H-NMR is a powerful approach of metabolomics. This study aimed to apply it to the profiling of serum metabolites in patients with pancreatitis, and to analyze the characteristic metabolites of pancreatitis.
Serum samples were taken from 17 pancreatitis patients and 23 healthy subjects and subjected to ¹H-NMR and principle component analysis to compare endogenous small-molecule metabolites.
3-hydroxybutyrate, trimethylamine-N-oxide, acetate and acetone levels were significantly lower in the pancreatitis group than in the control group. Isoleucine, acetylglycine, triglyceride and inosine levels were significantly higher in the pancreatitis group than in the control group.
¹H-NMR-based metabolomics is an effective method to investigate the small-molecule metabolites in the sera of patients with pancreatitis. Metabolites identified in this study may be exploited as metabolic markers for the early detection of pancreatitis.
背景/目的：氢核磁共振波谱（¹H-NMR）是代谢组学的一种强大方法。本研究旨在将其应用于胰腺炎患者血清代谢物的分析，并分析胰腺炎的特征性代谢物。
采集17例胰腺炎患者和23例健康受试者的血清样本，进行¹H-NMR和主成分分析，以比较内源性小分子代谢物。
胰腺炎组的3-羟基丁酸、氧化三甲胺、乙酸盐和丙酮水平显著低于对照组。胰腺炎组的异亮氨酸、乙酰甘氨酸、甘油三酯和肌苷水平显著高于对照组。
基于¹H-NMR的代谢组学是研究胰腺炎患者血清中小分子代谢物的有效方法。本研究中鉴定出的代谢物可作为胰腺炎早期检测的代谢标志物。
