De Simoni M G, De Luigi A, Imeri L, Algeri S
Istituto di-Ricerche Farmacologiche Mario Negri, Milano, Italia.
J Neurosci Methods. 1990 Aug;33(2-3):233-40. doi: 10.1016/0165-0270(90)90027-d.
In vivo voltammetry is an electrochemical technique that uses carbon fiber microelectrodes stereotaxically implanted in brain areas to monitor monoamine metabolism and release continuously, in freely moving animals. Electric wires connect the polarograph to the animal. A wire-less transmission system (optoelectronic transmission, OPT) of voltammetric signals is described here. It uses infrared diffused light, exploiting the diffusion of the transmitted light over walls and ceiling towards a receiver. The transmission system consists of a main unit and a satellite unit (40 x 30 x 5 mm) positioned on the animal's back. Voltammetric recordings obtained by the classical system (with wires) and by OPT are well defined and almost identical in shape. The power supply is provided by two thin lithium batteries (+/- 3V) that can record for up to 20 h. OPT permits detailed behavioral observations since the animal can be left free to move in a spacious environment. Voltammetry using OPT allows simultaneous recording of neuronal firing activity as well as electroencephalographic recordings (EEG) since there is no cross-talk between the circuits used. The results illustrate the reliability and usefulness of this wire-less transmission system for studying relationships between neurochemical, behavioral and electrophysiological activities.
体内伏安法是一种电化学技术，它使用立体定位植入脑区的碳纤维微电极，在自由活动的动物中连续监测单胺代谢和释放。电线将极谱仪与动物相连。本文描述了一种伏安信号的无线传输系统（光电传输，OPT）。它利用红外漫射光，通过墙壁和天花板将传输的光扩散到接收器。该传输系统由一个主机和一个位于动物背部的卫星单元（40×30×5毫米）组成。通过传统系统（有线）和OPT获得的伏安记录定义明确，形状几乎相同。由两块薄锂电池（±3V）供电，可记录长达20小时。OPT允许进行详细的行为观察，因为动物可以在宽敞的环境中自由活动。使用OPT的伏安法可以同时记录神经元放电活动以及脑电图记录（EEG），因为所用电路之间没有串扰。结果说明了这种无线传输系统在研究神经化学、行为和电生理活动之间关系方面的可靠性和实用性。
