Papazoglou Anna, Lundt Andreas, Wormuth Carola, Ehninger Dan, Henseler Christina, Soós Julien, Broich Karl, Weiergräber Marco
Department of Neuropsychopharmacology, Federal Institute for Drugs and Medical Devices (Bundesinstitut für Arzneimittel und Medizinprodukte, BfArM).
Molecular and Cellular Cognition Lab, German Center for Neurodegenerative Diseases (Deutsches Zentrum für Neurodegenerative Erkrankungen, DZNE).
J Vis Exp. 2016 Jun 25(112):54216. doi: 10.3791/54216.
Implantable EEG radiotelemetry is of central relevance in the neurological characterization of transgenic mouse models of neuropsychiatric and neurodegenerative diseases as well as epilepsies. This powerful technique does not only provide valuable insights into the underlying pathophysiological mechanisms, i.e., the etiopathogenesis of CNS related diseases, it also facilitates the development of new translational, i.e., therapeutic approaches. Whereas competing techniques that make use of recorder systems used in jackets or tethered systems suffer from their unphysiological restraining to semi-restraining character, radiotelemetric EEG recordings overcome these disadvantages. Technically, implantable EEG radiotelemetry allows for precise and highly sensitive measurement of epidural and deep, intracerebral EEGs under various physiological and pathophysiological conditions. First, we present a detailed protocol of a straight forward, successful, quick and efficient technique for epidural (surface) EEG recordings resulting in high-quality electrocorticograms. Second, we demonstrate how to implant deep, intracerebral EEG electrodes, e.g., in the hippocampus (electrohippocampogram). For both approaches, a computerized 3D stereotaxic electrode implantation system is used. The radiofrequency transmitter itself is implanted into a subcutaneous pouch in both mice and rats. Special attention also has to be paid to pre-, peri- and postoperative treatment of the experimental animals. Preoperative preparation of mice and rats, suitable anesthesia as well as postoperative treatment and pain management are described in detail.
可植入式脑电图无线电遥测技术在神经精神疾病、神经退行性疾病以及癫痫的转基因小鼠模型的神经学特征研究中具有核心意义。这项强大的技术不仅能为潜在的病理生理机制，即中枢神经系统相关疾病的病因发病机制提供有价值的见解，还能促进新的转化性治疗方法的开发。与利用夹克式记录仪系统或系留系统的竞争技术不同，这些技术存在非生理性限制到半限制特性的问题，而无线电遥测脑电图记录克服了这些缺点。从技术上讲，可植入式脑电图无线电遥测技术能够在各种生理和病理生理条件下精确且高度灵敏地测量硬膜外和深部脑内脑电图。首先，我们展示一种简单、成功、快速且高效的硬膜外（表面）脑电图记录技术的详细方案，该方案可得到高质量的皮质电图。其次，我们演示如何植入深部脑内脑电图电极，例如在海马体中植入（海马电图）。对于这两种方法，均使用计算机化三维立体定向电极植入系统。射频发射器本身植入小鼠和大鼠的皮下袋中。还必须特别注意实验动物的术前、术中和术后处理。详细描述了小鼠和大鼠的术前准备、合适的麻醉以及术后处理和疼痛管理。
