BOB Aesthetic Surgery Clinic, Seoul, Republic of Korea.
Curr Stem Cell Res Ther. 2012 May;7(3):165-72. doi: 10.2174/157488812799859847.
Human adipose tissue contains pluripotent stem cells that are similar to bone marrow-derived stem cells. The present study examined whether osteogenic induced adipose-derived stem cells (ASCs) could enhance the osteogenic capacity of demineralized bone matrix and accelerate bone formation in a rat critically-sized calvarial defect model.
Forty Sprague-Dawley rats were divided randomly into four groups containing 10 rats per each group (Control, 0.05 cc fibrin glue (25 mg/ml) and 0.05 cc thrombin (130 U/ml); DBX, control + 0.2 g DBX�; ASC, DBX + 1 x 105 ASCs/g; iASC, DBX + 1 x 105 osteogenic-induced ASCs/g). After osteogenic differentiation of ASCs, alkaline phosphatase and von Kossa staining were performed each week to determine the extent of differentiation and mineralization. An 8-mm critical size circular defect was made in the calvarial bone of each rat. The specimens were harvested 8 weeks after implantation, and radiographic and histological evaluations were carried out. New bone formation was quantified by radiodensitometric analysis of the calvarial sections. Statistical analysis was accomplished using a Mann-Whitney test and Kruskal-Wallis test at a significance level of P < 0.05.
Alkaline phosphatase and von Kossa staining showed that the osteogenic-induced ASCs yielded higher osteogenic differentiation at 3 weeks. The calvarial defect was filled more in the iASC group compared to the other groups, as demonstrated by the gross appearance of the specimen and radiologic evaluation. The mean radiodensity of the control, DBX, ASC, and iASC group was 16.78%, 39.94%, 25.58%, and 51.31%, respectively, and these were significantly different (P=0.034). Histomorphological evaluation confirmed that new bone formation was accelerated and enhanced by the osteogenic-induced ASCs.
ASCs produced greater osteogenic differentiation at 3 weeks. Osteogenic regeneration was accelerated and enhanced in vivo with the osteogenic-induced ASCs, compared to undifferentiated ASCs. Osteogenic-induced ASCs are an excellent and promising candidate for regenerative medicine and tissue engineering application.
人体脂肪组织中含有与骨髓来源的干细胞相似的多能干细胞。本研究旨在探讨成骨诱导脂肪源性干细胞（ASCs）是否能增强脱矿骨基质的成骨能力，并加速大鼠临界颅骨缺损模型中的骨形成。
40 只 Sprague-Dawley 大鼠随机分为四组，每组 10 只（对照组：0.05 cc 纤维蛋白胶（25mg/ml）和 0.05 cc 凝血酶（130U/ml）；DBX 组：对照组+0.2gDBX�；ASC 组：DBX+1x105 ASCs/g；iASC 组：DBX+1x105 成骨诱导 ASCs/g）。成骨诱导后，每周进行碱性磷酸酶和 von Kossa 染色，以确定分化和矿化程度。在每只大鼠颅骨上制作 8mm 圆形临界大小缺损。植入 8 周后取出标本，进行放射学和组织学评估。通过对颅骨切片的放射密度分析来定量新骨形成。使用 Mann-Whitney 检验和 Kruskal-Wallis 检验进行统计学分析，显著性水平为 P<0.05。
碱性磷酸酶和 von Kossa 染色显示，成骨诱导的 ASCs 在第 3 周时产生更高的成骨分化。与其他组相比，iASC 组的颅骨缺损填充更多，这可以从标本的大体外观和放射学评估中看出。对照组、DBX 组、ASC 组和 iASC 组的平均放射密度分别为 16.78%、39.94%、25.58%和 51.31%，差异有统计学意义（P=0.034）。组织形态学评价证实，成骨诱导的 ASCs 加速和增强了新骨形成。
ASCs 在第 3 周时产生更大的成骨分化。与未分化的 ASCs 相比，成骨诱导的 ASCs 在体内加速和增强了成骨再生。成骨诱导的 ASCs 是再生医学和组织工程应用的优秀且有前途的候选物。
