Department of Plastic and Reconstructive Surgery, Inje University Ilsan Paik Hospital, Goyang City, Gyeonggi-do, Republic of Korea.
Stem Cells Dev. 2011 Feb;20(2):233-42. doi: 10.1089/scd.2009.0525. Epub 2010 Oct 12.
Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX(®)) and/or poly(70L-lactide-co-30DL-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM + SVF, (4) DBM + PLA, and (5) DBM + PLA + SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM + SVF and DBM + PLA + SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM + SVF: 57.69%; DBM + PLA: 24.86%; DBM + PLA + SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM + SVF and DBM + PLA + SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.
虽然许多研究表明人类脂肪组织中含有多能干细胞，但关于基质血管部分（SVF）的报道很少。在本研究中，我们评估了 SVF 的成骨能力。我们植入未经培养的新鲜分离的脂肪源性干细胞与脱钙骨基质（DBM）结合，以诱导临界大小大鼠颅骨缺损模型中的骨再生。我们使用 DBM（DBX®）和/或聚（70L-乳酸-co-30DL-乳酸）共聚物 PLA 作为支架。将 50 只白色大鼠随机分为 5 个不同组（n=10）：（1）对照组，（2）DBM 组，（3）DBM+SVF 组，（4）DBM+PLA 组和（5）DBM+PLA+SVF 组。在获得 SVF 后，在每只大鼠中制作一个 8mm 的临界大小颅骨缺损。植入后 8 周采集标本，进行影像学和组织学评估。通过苏木精和伊红染色以及颅骨切片的抗骨钙素抗体（OC4-30）免疫染色定性新骨形成。通过放射密度测量分析确定矿化量。大体观察，DBM+SVF 和 DBM+PLA+SVF 组的骨形成量多于其他组。放射密度测量评估显示，根据 Kruskal-Wallis（秩）检验（P=0.030<0.05），各组间存在显著差异。5 组骨缺损的填充量不同（对照组：13.48%；DBM 组：39.94%；DBM+SVF 组：57.69%；DBM+PLA 组：24.86%；DBM+PLA+SVF 组：42.75%）。组织学评价显示，DBM+SVF 和 DBM+PLA+SVF 组有丰富的新骨形成。结果发现，SVF 形式的未分化脂肪源性干细胞诱导大鼠颅骨缺损中的新骨形成。因此，SVF 为再生组织工程或细胞为基础的治疗提供了一种实用、有前途的候选物。
