Dipartimento di Science Cliniche, Facoltà di Medicina, Università Politecnica delle Marche, Italy.
Food Chem Toxicol. 2012 May;50(5):1508-16. doi: 10.1016/j.fct.2012.01.042. Epub 2012 Feb 4.
The aim of the present work was to analyze the phenolic extracts from two monofloral Cuban honeys for their in vitro total antioxidant capacity, phenolic compounds content and free radical scavenging activity. The phenolic extracts, rich in lipophilic compounds, were tested further for their ability to inhibit AAPH-induced oxidative damage (hemolysis, lipid peroxidation and cytosolic depletion of reduced glutathione and decrease of superoxide dismutase activity) in erythrocytes. Results indicate an important total antioxidant capacity measured by TEAC and ORAC assays, as well as a relevant radical scavenging activity performed by EPR. Moreover, 13 phenolic compounds were identified using HPLC-LC/MS with quercetin as the most abundant flavonoid. The results also show that both extracts were able to inhibit erythrocytes oxidative damage, and that this may likely be due to their incorporation into cell membranes and their ability to cross it and reach the cytosol. In fact, flavonoid uptake by erythrocytes was further confirmed by testing quercetin, which efficiently incorporated into erythrocytes. Overall, this study indicates that honey contains relevant antioxidant compounds responsible, at least in part, for its biological activity and that uptake of its flavonoids may provide defense and promote cell functions in erythrocytes.
本研究旨在分析来自古巴两种单花种蜂蜜的酚类提取物的体外总抗氧化能力、酚类化合物含量和自由基清除活性。这些富含亲脂性化合物的酚类提取物进一步被测试其抑制 AAPH 诱导的红细胞氧化损伤（溶血、脂质过氧化、细胞内还原型谷胱甘肽耗竭以及超氧化物歧化酶活性降低）的能力。结果表明，TEAC 和 ORAC 测定法测定的总抗氧化能力以及 EPR 测定的自由基清除活性均较高。此外，使用 HPLC-LC/MS 鉴定出 13 种酚类化合物，其中槲皮素是最丰富的类黄酮。研究结果还表明，两种提取物均能抑制红细胞氧化损伤，这可能归因于其进入细胞膜并穿过细胞膜进入细胞质的能力。事实上，通过测试槲皮素进一步证实了黄酮类化合物被红细胞摄取，槲皮素有效地被摄取到红细胞中。总的来说，本研究表明蜂蜜中含有相关的抗氧化化合物，这些化合物至少部分负责其生物活性，并且其类黄酮的摄取可能为红细胞提供防御并促进其细胞功能。
