Department of Biochemistry, Faculty of Medicine, Marche Polytechnic University, Via Ranieri 65, 60100 Ancona, Italy.
Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2490-9. doi: 10.1016/j.fct.2010.06.021. Epub 2010 Jun 15.
Several monofloral Cuban honeys were analyzed to determine their total phenolic, flavonoid, ascorbic acid, amino acid, protein and carotenoid contents as well as their radical-scavenging activity and antimicrobial capacities. The total phenolic, flavonoid and carotenoid contents varied considerably, and the highest values were obtained for Linen vine (Govania polygama (Jack) Urb) honey, which is classified as an amber honey. The highest amino acid content was found in Morning glory (Ipomoea triloba L.) while Liven vine had the highest protein content. Similarly Linen vine honey had the highest antioxidant activity while the lowest was found in Christmas vine (Turbina corymbosa (L.) Raf). Ascorbic acid was absent. Hydroxyl radical formation was studied by EPR and spin trapping, and it was found in all honeys tested. The antimicrobial activity was screened using two Gram-positive and Gram-negative bacteria. S. aureus was the most sensitive microorganism while Pseudomonas aeruginosa presented higher minimum active dilution values. Bacillus subtilis and Escherichia coli were both moderately sensitive to honey antimicrobial activity. A correlation between radical-scavenging activity and total phenolic content was found. Correlation existed also between color vs phenolics content, vs flavonoid content or between phenolic vs flavonoid.
几种古巴单花蜜进行了分析，以确定其总酚、类黄酮、抗坏血酸、氨基酸、蛋白质和类胡萝卜素含量，以及它们的自由基清除活性和抗菌能力。总酚、类黄酮和类胡萝卜素含量差异很大，亚麻藤（Govania polygama (Jack) Urb）蜂蜜的含量最高，被归类为琥珀色蜂蜜。天门冬氨酸含量最高的是旋花（Ipomoea triloba L.），而亚麻藤的蛋白质含量最高。同样，亚麻藤蜂蜜的抗氧化活性最高，圣诞藤（Turbina corymbosa (L.) Raf）的抗氧化活性最低。抗坏血酸不存在。通过 EPR 和自旋捕获研究了羟基自由基的形成，发现所有测试的蜂蜜中都存在这种自由基。使用两种革兰氏阳性菌和革兰氏阴性菌对其抗菌活性进行了筛选。金黄色葡萄球菌是最敏感的微生物，铜绿假单胞菌的最小有效稀释值较高。枯草芽孢杆菌和大肠杆菌对蜂蜜的抗菌活性都有中等的敏感性。发现自由基清除活性与总酚含量之间存在相关性。颜色与酚类含量、黄酮类含量之间也存在相关性，或者酚类与黄酮类之间也存在相关性。
