Michigoshi Yuuki, Yamagishi Norio, Satoh Hiroshi, Kato Masaki, Furuhama Kazuhisa
Department of Veterinary Basic Medicine, Iwate University, Morioka, Japan.
J Am Assoc Lab Anim Sci. 2011 Sep;50(5):702-7.
To establish a simple procedure for estimating the glomerular filtration rate (GFR) in conscious rabbits, we used the conventional multisample approach to develop a single-blood-sample method. A bolus injection of inulin was administered intravenously at a dose of 40 mg/kg to male New Zealand White rabbits, and blood was collected 30, 60, 90, and 120 min later. Serum inulin, urea nitrogen, and creatinine concentrations were determined. Using this multi-sample method, the reference GFR in clinically healthy rabbits was 4.01 ± 0.17 mL/min/kg (n = 17). In rabbits given an intravenous injection of the antitumor agent cisplatin, GFR fell before serum urea nitrogen and creatinine concentrations increased. Based on cumulative GFR data from healthy and nephropathy rabbits, the GFR obtained from the 3-sample method (30-, 60-, and 90-min samples) was closely correlated (r = 0.99) with that calculated from the estimated distribution volume and serum inulin concentration at 90 min after inulin injection in the single-blood-sample method. These results demonstrate that the single-blood-sample method supports sequential GFR measurements in rabbits and is a versatile procedure not only for research purposes but also in clinical settings.
为建立一种估算清醒家兔肾小球滤过率（GFR）的简单方法，我们采用传统的多样本方法开发了一种单血样法。以40 mg/kg的剂量向雄性新西兰白兔静脉推注菊粉，然后在30、60、90和120分钟后采集血液。测定血清菊粉、尿素氮和肌酐浓度。使用这种多样本法，临床健康家兔的参考GFR为4.01±0.17 mL/min/kg（n = 17）。在静脉注射抗肿瘤药物顺铂的家兔中，GFR在血清尿素氮和肌酐浓度升高之前就下降了。基于健康家兔和肾病家兔的累积GFR数据，多样本法（30、60和90分钟样本）得到的GFR与单血样法中菊粉注射后90分钟根据估算分布容积和血清菊粉浓度计算得到的GFR密切相关（r = 0.99）。这些结果表明，单血样法支持对家兔进行连续的GFR测量，并且不仅是一种适用于研究目的的通用方法，也适用于临床环境。
