Seidmon E J, Krisch E B, Baer H M, Phillips S J, Tang C K, Shea F J
Department of Urology, Temple University Hospital, Philadelphia, PA.
Lasers Surg Med. 1990;10(5):433-7. doi: 10.1002/lsm.1900100506.
This is the second study of a two-prong investigation conducted to learn two different aspects of vasovasostomy in dog vas deferens by using the carbon dioxide milliwatt laser. This investigation involves the evaluation of patency and analysis of sperm. Six dogs underwent vasectomy, followed by vasovasostomies performed between 4 and 12 weeks later, utilizing two approximating sutures and welding with the carbon dioxide milliwatt laser. Metal clips were used both for x-ray analysis and localization of the vasovasostomy site. All dogs had pre-vasectomy semen analysis. Following vasectomy each dog underwent ejaculation until azoospermia was obtained. Vasovasostomy was performed and semen was examined. Prior to harvesting, vasography was also obtained. All ejaculates had active sperm noted and 12 of 12 vasograms (100%) revealed patency. The dogs were sacrificed at 4 weeks, 6 weeks, and 8 weeks post-vasovasostomy. The vasovasostomy specimens were studied with electron microscopy and by histological evaluation. The vasovasostomy was completed in under 90 minutes by using this laser-assisted technique. This procedure offers to the urologist a more simplified and reproducible operation that can be performed easily and with a potential decrease in hospital costs.
这是一项双管齐下研究的第二项研究，该研究旨在通过使用二氧化碳毫瓦激光了解犬输精管吻合术的两个不同方面。这项研究包括对通畅性的评估和精子分析。六只狗接受了输精管切除术，然后在4至12周后进行输精管吻合术，使用两根近似缝线并用二氧化碳毫瓦激光进行焊接。金属夹用于X射线分析和输精管吻合术部位的定位。所有狗在输精管切除术前都进行了精液分析。输精管切除术后，每只狗进行射精，直到获得无精子症。进行输精管吻合术并检查精液。在采集之前，还进行了输精管造影。所有射精都发现有活动精子，12份输精管造影中有12份（100%）显示通畅。这些狗在输精管吻合术后4周、6周和8周被处死。对输精管吻合术标本进行了电子显微镜和组织学评估。使用这种激光辅助技术，输精管吻合术在90分钟内完成。该手术为泌尿科医生提供了一种更简化、可重复的手术，操作简便，可能降低医院成本。
