Laboratório de Genética Toxicológica, Universidade Luterana do Brasil, Canoas, RS, Brazil.
Mutat Res. 2012 May 15;744(2):140-4. doi: 10.1016/j.mrgentox.2012.01.008. Epub 2012 Feb 6.
Nicotine has been reported to cause acute toxicity and to present long-term risks, such as chromosomal damage and genetic instability. The genotoxicity of nicotine may be mediated partly by an oxidative mechanism. We have evaluated the effects of the antioxidant vitamin C on nicotine-induced genotoxicity in mice. The comet assay and the micronucleus test were used to assess the effects of nicotine (15mg/kg) at different exposure times (2, 4, and 24h in the comet assay; 24h in the micronucleus test). Pretreatment with vitamin C 24h before nicotine exposure strongly protected mice against nicotine-induced DNA damage.
尼古丁已被报道会引起急性毒性，并带来长期风险，如染色体损伤和遗传不稳定性。尼古丁的遗传毒性可能部分通过氧化机制介导。我们评估了抗氧化维生素 C 对尼古丁诱导的小鼠遗传毒性的影响。彗星试验和微核试验用于评估不同暴露时间（彗星试验中 2、4 和 24 小时；微核试验中 24 小时）的尼古丁（15mg/kg）的影响。在尼古丁暴露前 24 小时用维生素 C 预处理可强烈保护小鼠免受尼古丁引起的 DNA 损伤。
