利用高极化 [1-(13)C]丙酮酸快速容积成像技术研究大鼠肝脏中的乙醇代谢。
Fast volumetric imaging of ethanol metabolism in rat liver with hyperpolarized [1-(13) C]pyruvate.
机构信息
SRI International, Neuroscience Program, Menlo Park, CA 94025, USA.
出版信息
NMR Biomed. 2012 Aug;25(8):993-9. doi: 10.1002/nbm.2762. Epub 2012 Feb 14.
Abstract
Rapid volumetric imaging of hyperpolarized (13) C compounds allows the real-time measurement of metabolic activity and can be useful in distinguishing between normal and diseased tissues. This work extends a fast two-dimensional undersampled spiral MRSI sequence to provide volumetric coverage, acquiring a 16 × 16 × 12 matrix with a nominal isotropic resolution of 5 mm in 4.5 s. The rapid acquisition enables a high temporal resolution for dynamic imaging. This dynamic three-dimensional MRSI method was used to investigate hyperpolarized [1-(13) C]pyruvate metabolism modulated by the administration of ethanol in rat liver. A significant increase in the pyruvate to lactate conversion was observed in the liver as a result of the greater availability of NADH (nicotinamide adenine dinucleotide, reduced form) from ethanol metabolism.
摘要
利用（13）C 标记的极化化合物进行快速容积成像，可以实时测量代谢活性，并有助于区分正常组织和病变组织。这项工作扩展了一种快速二维欠采样螺旋 MRSI 序列，以提供容积覆盖，在 4.5 秒内采集具有标称各向同性分辨率 5mm 的 16×16×12 矩阵。快速采集能够实现高时间分辨率的动态成像。该动态三维 MRSI 方法用于研究在大鼠肝脏中通过给予乙醇调节的 [1-（13）C]丙酮酸代谢。由于来自乙醇代谢的 NADH（烟酰胺腺嘌呤二核苷酸，还原型）的可用性增加，观察到肝脏中丙酮酸向乳酸的转化率显著增加。
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