Sokolova O I, Dem'ianov A V, Bovers L S, Did'e E S, Sokolova Iu Ia
Tsitologiia. 2011;53(11):911-4.
The FTA technology was applied for sampling, archiving, and molecular analysis of the DNA isolated from stool samples to diagnose and identify microsporidia, the intracellular opportunistic parasites which induce malabsortion syndrome in immunosuppressed humans, particularly in patients with AIDS. Microsporidia DNA was successfully amplified in 6 of 50 stool samples of HIV-positive patients of the S. P. Botkin Memorial Infectious Disease Hospital (St. Petersburg) applied to FTA cards (FTA-Cars, Whatman Inc. Florham Park, NJ, USA). Amplicons (the fragments of rDNA) were directly sequenced, and microsporidia species--Encephalitozoon intestinalis, E. cuniculi, E. hellem, and Enterocytozoon bieneusi--were identified in Genbank by NCBI BLAST program. The FTA method of DNA immobilization is especially promising for epidemiological and field population studies which involve genotyping of microsporidia species and isolates.
FTA技术被应用于从粪便样本中分离DNA的采样、存档及分子分析，以诊断和鉴定微孢子虫，这种细胞内机会性寄生虫会在免疫抑制人群中引发吸收不良综合征，尤其是艾滋病患者。圣彼得堡的S.P.博特金纪念传染病医院的50例HIV阳性患者的粪便样本中有6例成功应用FTA卡（FTA-Cars，美国新泽西州弗洛勒姆帕克的Whatman公司）分离出微孢子虫DNA。对扩增产物（rDNA片段）进行直接测序，并通过NCBI BLAST程序在Genbank中鉴定出微孢子虫种类——肠脑炎微孢子虫、兔脑炎微孢子虫、海伦脑炎微孢子虫和比氏肠细胞内微孢子虫。DNA固定的FTA方法对于涉及微孢子虫种类和分离株基因分型的流行病学和现场人群研究尤其有前景。
