UCD Obstetrics & Gynaecology, School of Medicine and Medical Science, University College Dublin, National Maternity Hospital, Dublin, Ireland.
Gynecol Endocrinol. 2012 Sep;28(9):682-5. doi: 10.3109/09513590.2011.652245. Epub 2012 Feb 14.
Cocaine- and amphetamine-regulated transcript (CART) is a leptin-regulated anorectic neuropeptide. Increased levels of leptin in cord blood of diabetic mothers have previously been described. The aim of this study was to quantify maternal and fetal serum CART levels in type 1 diabetes mellitus (T1DM, n = 10) and non-diabetic pregnancy (n = 10). Matched maternal serum samples (n = 20) were obtained at 36-weeks gestation and cord samples from the umbilical vein at delivery (n = 20), CART was quantified using a competitive enzyme immunoassay. Statistical analysis was performed using Spearmans correlation and t test. There was no difference in maternal CART levels at 36-weeks gestation between T1DM (mean = 331.13 pg/ml, Standard Error of the Mean (SEM) = 114.54) and non-diabetic pregnancy (mean = 195.01 pg/ml SEM = 29.37) (p = 0.106). Fetal CART levels in the umbilical vein were similar in T1DM (mean = 199.27 pg/ml, SEM = 39.81) and non-diabetic pregnancy (mean = 149.76 pg/ml, SEM = 26.08) (p = 0.143). Maternal serum CART levels measured at 36-weeks gestation correlated with maternal BMI at booking (Spearmans ρ = 0.332) (p = 0.001) irrespective of diabetes. Serum CART can be detected in both diabetic and non-diabetic human pregnancy and may play an important role in body mass regulation in pregnancy.
可卡因-和苯丙胺调节转录物（CART）是一种瘦素调节的厌食神经肽。先前已经描述了糖尿病母亲脐带血中瘦素水平升高。本研究的目的是定量测定 1 型糖尿病（T1DM，n=10）和非糖尿病妊娠（n=10）中母血清和胎儿血清 CART 水平。在 36 周妊娠时获得匹配的母血清样本（n=20），分娩时从脐静脉获得脐带血样本（n=20），使用竞争性酶免疫测定法定量测定 CART。使用 Spearman 相关和 t 检验进行统计分析。T1DM 组（均值=331.13pg/ml，标准误均值（SEM）=114.54）和非糖尿病妊娠组（均值=195.01pg/ml SEM=29.37）在 36 周妊娠时的母血清 CART 水平没有差异（p=0.106）。T1DM 组（均值=199.27pg/ml，SEM=39.81）和非糖尿病妊娠组（均值=149.76pg/ml，SEM=26.08）胎儿脐静脉 CART 水平相似（p=0.143）。在 36 周妊娠时测量的母血清 CART 水平与母本初诊时的 BMI 相关（Spearman ρ=0.332）（p=0.001），与糖尿病无关。CART 可在糖尿病和非糖尿病的人类妊娠中检测到，可能在妊娠期间的体重调节中发挥重要作用。
