Department of Physical Therapy, Federal University of São Carlos, São Carlos, Brazil.
Clin Exp Pharmacol Physiol. 2012 May;39(5):417-22. doi: 10.1111/j.1440-1681.2012.05690.x.
Myostatin (MSTN) has been implicated in metabolic adaptation to physiological stimuli, such as physical exercise, which is linked to improved glucose homeostasis. The aim of the present study was to evaluate the influence of exercise on the expression of MSTN, MSTN receptors (ActRIIB and ALK4) and follistatin (FS) in the muscle and fat of streptozotocin-induced diabetic rats. Control and diabetic rats were randomly assigned to a swimming training group (EC and ED, respectively) and a sedentary group (SC and SD, respectively). Exercising animals swam for 45 min at 0900 and 1700 hours, 5 day/week, for 4 weeks. The mRNA expression of MSTN, ActRIIB, ALK4 and FS mRNA was quantified by real-time reverse transcription-polymerase chain reaction. Expression of MSTN and FS mRNA increased in the muscle and subcutaneous fat of SD compared with SC rats. Expression of ActRIIB mRNA was increased in the muscle, mesenteric fat and brown adipose tissue (BAT) of SD compared with SC rats, whereas ALK4 mRNA expression was only increased in the BAT of SD compared with SC rats. After training, MSTN and ActRIIB expression was lower in the BAT of EC compared with SC rats. Expression of MSTN mRNA increased in the mesenteric fat of ED compared with SD rats, whereas FS mRNA expression decreased in the muscle, mesenteric and subcutaneous fat and BAT. Lower ALK4 mRNA expression was noted in the BAT of ED compared with SD rats. These results indicate that MSTN, its receptors and FS expression change in both the muscle and fat of diabetic rats and that the expression of these factors can be modulated by exercise in diabetes.
肌肉生长抑制素 (MSTN) 已被牵涉到生理刺激（如体育锻炼）的代谢适应中，这与改善葡萄糖稳态有关。本研究的目的是评估运动对链脲佐菌素诱导的糖尿病大鼠肌肉和脂肪中 MSTN、MSTN 受体（ActRIIB 和 ALK4）和卵泡抑素 (FS) 表达的影响。对照组和糖尿病组大鼠被随机分为游泳训练组（EC 和 ED）和久坐组（SC 和 SD）。运动组动物每天上午 9 点和下午 5 点游泳 45 分钟，每周 5 天，持续 4 周。通过实时逆转录聚合酶链反应定量检测 MSTN、ActRIIB、ALK4 和 FS mRNA 的表达。与 SC 组相比，SD 组大鼠的肌肉和皮下脂肪中 MSTN 和 FS mRNA 的表达增加。与 SC 组相比，SD 组大鼠的肌肉、肠系膜脂肪和棕色脂肪组织（BAT）中 ActRIIB mRNA 的表达增加，而 ALK4 mRNA 的表达仅在 SD 组大鼠的 BAT 中增加。训练后，EC 组大鼠的 BAT 中 MSTN 和 ActRIIB 的表达低于 SC 组。ED 组大鼠的肠系膜脂肪中 MSTN mRNA 的表达增加，而肌肉、肠系膜和皮下脂肪以及 BAT 中 FS mRNA 的表达减少。ED 组大鼠的 BAT 中 ALK4 mRNA 的表达降低。这些结果表明，糖尿病大鼠的肌肉和脂肪中 MSTN、其受体和 FS 的表达发生变化，并且这些因素的表达可以通过运动在糖尿病中进行调节。
