Department of Pharmaceutical Products, Faculty of Pharmacy, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Mar 1;887-888:133-7. doi: 10.1016/j.jchromb.2012.01.009. Epub 2012 Jan 25.
Cylitol L-(+)-bornesitol is regarded as a bioactive constituent from Hancornia speciosa leaves, a plant species traditionally used in Brazil to treat diabetes and hypertension. We report a simple HPLC-DAD method for the quantification of bornesitol in extracts from H. speciosa leaves, after derivatization with p-toluenesulfonyl chloride, using pentaerythritol as internal standard. A gradient of methanol, acetonitrile and water was employed for elution on an ODS column and detection was set up at a wavelength of 230 nm. The method was selective and linear over the range 60.4-302.0 μg/ml with r² of 0.9981, and showed satisfactory precision for intra-day (RSD=2.37%) and inter-day (RSD=3.17%) assays. The recovery varied between 92.3% and 99.9% and the limits of quantification and detection were respectively 5.00 and 1.67 μg/ml. The method was applied to quantify bornesitol in extracts from H. speciosa leaves of different specimens.
左旋异丁子香酚（Cylitol L-(+)-bornesitol）被认为是巴西传统用于治疗糖尿病和高血压的药用植物龙舌兰（Hancornia speciosa）叶子中的一种生物活性成分。我们报道了一种简单的 HPLC-DAD 方法，用于衍生化后采用内标法（戊四醇）通过对甲苯磺酰氯定量检测龙舌兰叶子提取物中的异丁子香酚。在 ODS 柱上采用甲醇、乙腈和水的梯度洗脱，检测波长为 230nm。该方法选择性好，线性范围为 60.4-302.0μg/ml，r²为 0.9981，日内（RSD=2.37%）和日间（RSD=3.17%）测定的精密度均令人满意。回收率在 92.3%至 99.9%之间，定量限和检测限分别为 5.00 和 1.67μg/ml。该方法应用于不同样本龙舌兰叶子提取物中异丁子香酚的定量检测。
