Department of Pharmaceutical Sciences, Center of Health Sciences, Universidade Federal de Santa Catarina, Campus Trindade, Florianópolis, SC, 88.040-900, Brazil.
Department of Pharmaceutical Products, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Phytother Res. 2015 Dec;29(12):1887-93. doi: 10.1002/ptr.5438. Epub 2015 Sep 6.
The leaves of Hancornia speciosa Gomes (Apocynaceae), a medicinal species found in the Brazilian cerrado biome, are traditionally used to treat wounds and inflammatory disorders. The goal of the present study was to investigate the in vitro wound healing properties of ethanolic extract of H. speciosa leaves and its isolated compounds, using the scratch assay, and to evaluate their effects on the release of the pro-inflammatory cytokine tumor necrosis factor (TNF-α) by lipopolysaccharide (LPS)-stimulated human acute monocytic (THP-1) cells. H. speciosa ethanolic extract significantly increased (42.8% ± 5.4 at 25 µg/mL) cell migration and proliferation of fibroblasts compared with control cells, as well as the isolated compounds bornesitol (80.8% ± 5.1) and quinic acid (69.1% ± 6.2), both assayed at 50 μM. TNF-α release by LPS-stimulated THP-1 cells was significantly reduced by the ethanolic extract (62.9% ± 8.2, i.e. 1791.1 ± 394.7 pg/mL) at 10 µg/mL, bornesitol (48.9% ± 0.9, i.e. 2461.6 ± 43.1 pg/mL) at 50 μM, and quinic acid (90.2% ± 3.4, i.e. 473.5 ± 164.4 pg/mL) and rutin (82.4% ± 5.6, i.e. 847.0 ± 271.8 pg/mL) at 10 μM. These results provided evidences to support the traditional use of H. speciosa leaves to treat wounds and inflammatory disorders.
亚马孙奶树（夹竹桃科）的叶子是一种药用植物，生长在巴西塞拉多生物群系，传统上用于治疗伤口和炎症性疾病。本研究的目的是使用划痕实验研究亚马孙奶树叶的乙醇提取物及其分离化合物的体外伤口愈合特性，并评估它们对脂多糖（LPS）刺激的人急性单核细胞（THP-1）细胞释放促炎细胞因子肿瘤坏死因子（TNF-α）的影响。与对照细胞相比，亚马孙奶树乙醇提取物显著增加（25μg/mL 时为 42.8%±5.4）成纤维细胞的迁移和增殖，以及分离化合物borne-sitol（50μM 时为 80.8%±5.1）和奎尼酸（69.1%±6.2）。LPS 刺激的 THP-1 细胞释放的 TNF-α 被乙醇提取物（10μg/mL 时为 62.9%±8.2，即 1791.1±394.7pg/mL）、borne-sitol（50μM 时为 48.9%±0.9，即 2461.6±43.1pg/mL）、奎尼酸（10μM 时为 90.2%±3.4，即 473.5±164.4pg/mL）和芦丁（82.4%±5.6，即 847.0±271.8pg/mL）显著降低。这些结果为亚马孙奶树叶子用于治疗伤口和炎症性疾病的传统用途提供了证据支持。
