State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China.
Analyst. 2012 Apr 7;137(7):1669-73. doi: 10.1039/c2an15983j. Epub 2012 Feb 15.
In this work, two different deposition methods of 3-aminopropyltriethoxysilane (APTES) on glass slides were compared in order to study the adhesion effect of cervical exfoliated cells on smear slides. Glass slides were modified by vapor-phase deposition (V-D) and liquid-phase deposition (L-D), respectively. The topographic images and amine density of the modified slides were investigated by using atomic force microscopy, UV-vis spectroscopy and X-ray photoelectron spectroscopy. The numbers of cells adhered on the slides functionalized by V-D and L-D were counted and compared under the microscope. The data showed significant differences between the two methods (t-test: P < 0.05). The results presented here have made it theoretically possible to produce amine slides by V-D method for the ThinPrep cytologic test.
本工作比较了两种不同的 3-氨丙基三乙氧基硅烷（APTES）在载玻片上的沉积方法，以研究宫颈脱落细胞在涂片上的黏附效果。载玻片分别通过气相沉积（V-D）和液相沉积（L-D）进行改性。通过原子力显微镜、紫外可见光谱和 X 射线光电子能谱研究了改性玻片的形貌图像和胺密度。在显微镜下计数并比较了经 V-D 和 L-D 功能化的玻片上黏附的细胞数量。数据显示两种方法之间存在显著差异（t 检验：P < 0.05）。本研究结果从理论上为 ThinPrep 细胞学检测中通过 V-D 方法制备胺基玻片提供了可能。
