Institute of Pathology, University Medical Centre Hamburg-Eppendorf, Martinistrasse 52, Hamburg, Germany.
Histopathology. 2012 Jun;60(7):1099-106. doi: 10.1111/j.1365-2559.2011.04152.x. Epub 2012 Feb 15.
Cellular areas of chondroblastoma are composed of polygonal chondroblasts with indented nuclei and scattered osteoclast-type multinucleated cells. To learn more about the phenotype of chondroblasts, we investigated the expression of several established immunohistochemical markers in chondroblastomas.
Nine chondroblastomas were analysed using immunohistochemical antibodies [CD34, α-smooth muscle actin (α-SMA), DOG1, CD117, AE1/AE3 and CD163]. Ten chondromyxoid fibromas, seven giant cell tumours of bone and four foetal proximal femurs were also analysed. The cellular areas of each chondroblastoma contained nests of DOG1(+) αSMA(+) CD117(-) CD34(-) chondroblasts, a phenotype that was not detected in chondromyxoid fibroma cases or in giant cell tumours. Although AE1/AE3 was expressed in all chondroblastomas, the staining intensity and proportion of the positive cells varied widely. Intra-lesional CD163(+) macrophages were detected in all cases of chondroblastoma, chondromyxoid fibroma and giant cell tumours.
Our results demonstrated nests of membranous DOG1(+) chondroblasts located within cellular portions of chondroblastoma containing diffuse heterogeneous infiltrates of mostly DOG1(-) chondroblasts, CD163(+) macrophages and multinucleated osteoclastic giant cells. Thus, chondroblastoma can be added to the tumours that are usually positive for DOG1, alongside gastrointestinal stromal tumour (GIST), rare solid-pseudopapillary neoplasms of the pancreas and exceptional mesenchymal tumours including uterine type retroperitoneal leiomyoma, peritoneal leiomyomatosis and synovial sarcoma.
成软骨细胞瘤的细胞区由具有凹陷核的多边形成软骨细胞和散在的破骨细胞样多核细胞组成。为了更多地了解成软骨细胞的表型，我们研究了成软骨细胞瘤中几种已建立的免疫组织化学标志物的表达。
使用免疫组织化学抗体[CD34、α-平滑肌肌动蛋白（α-SMA）、DOG1、CD117、AE1/AE3 和 CD163]分析了 9 例成软骨细胞瘤。还分析了 10 例软骨黏液样纤维瘤、7 例骨巨细胞瘤和 4 例胎儿股骨近端。每个成软骨细胞瘤的细胞区均包含 DOG1(+)α-SMA(+)CD117(-)CD34(-)的成软骨细胞巢，这种表型未在软骨黏液样纤维瘤病例或骨巨细胞瘤中检测到。虽然 AE1/AE3 在所有成软骨细胞瘤中均有表达，但染色强度和阳性细胞的比例差异很大。所有成软骨细胞瘤、软骨黏液样纤维瘤和骨巨细胞瘤中均检测到内瘤 CD163(+)巨噬细胞。
我们的结果表明，DOG1(+)的膜性成软骨细胞巢位于成软骨细胞瘤的细胞区内部，这些细胞区包含弥漫性异质性浸润，主要为 DOG1(-)的成软骨细胞、CD163(+)巨噬细胞和多核破骨细胞样巨细胞。因此，成软骨细胞瘤可以与胃肠道间质瘤（GIST）、胰腺罕见的实性假乳头状肿瘤以及包括子宫型腹膜后平滑肌瘤、腹膜平滑肌瘤病和滑膜肉瘤在内的罕见间叶肿瘤一起，归入通常为 DOG1 阳性的肿瘤之列。
