School of Earth and Environmental Sciences, College of Natural Sciences, Seoul National University, Seoul, 151-747, Korea.
J Eukaryot Microbiol. 2012 Mar-Apr;59(2):114-33. doi: 10.1111/j.1550-7408.2012.00610.x. Epub 2012 Feb 15.
Coolia spp. are epiphytic and benthic dinoflagellates. Herein, we report for the first time, the occurrence of Coolia canariensis and Coolia malayensis in Korean waters. The morphology of the Korean strains of C. canariensis and C. malayensis isolated from the waters off Jeju Island, Korea was similar to that of the original Canary lslands strains and Malaysian strains, respectively. We found several pores and a line of small knobs on the pore plate, and perforations within the large pores of both C. canariensis and C. malayensis. The plates of the Korean strains of C. canariensis and C. malayensis were arranged in a Kofoidian series of Po, 3', 7'', 6c, 6s, 5''', and 2'''', and Po, 3', 7'', 7c, 6-7s, 5''', and 2'''', respectively. When properly aligned, the large subunit (LSU) rDNA sequence of the Korean strain of C. canariensis was identical to that of the Biscayan strains, but it was 2-3% different from the Canary lslands strain VGO0775 and the Australian strain. In addition, the sequences of small subunit (SSU) and/or LSU rDNA from the two Korean strains of C. malayensis were < 1% different from the Malaysian strains of C. malayensis and the Florida strain CCMP1345 and New Zealand strain CAWD39 ("Coolia monotis"). In phylogenetic trees based on LSU rDNA sequences, the Korean strains of C. malayensis belonged to a clade including the Malaysian strains and these two strains. Therefore, based on genealogical analyses, we suggest that the Korean strain of C. canariensis is closely related to two Atlantic strains and the Australian strain, whereas the Korean strains of C. malayensis are related to the Malaysian strains of C. malayensis and the Florida and New Zealand strains.
冷鞭毛藻属物种是附生和底栖的双鞭毛藻类。本研究首次在韩国水域中发现了卡氏冷鞭毛藻和马来西亚冷鞭毛藻。从韩国济州岛海域分离出的韩国冷鞭毛藻和马来西亚冷鞭毛藻的形态与原加那利群岛株和马来西亚株相似。我们在孔板上发现了几个孔和一行小瘤，在卡氏冷鞭毛藻和马来西亚冷鞭毛藻的大孔内发现了穿孔。韩国冷鞭毛藻和马来西亚冷鞭毛藻的藻体盘呈 Kofoidian 系列排列，Po、3'、7''、6c、6s、5'''和 2'''，以及 Po、3'、7''、7c、6-7s、5'''和 2'''。当排列正确时，韩国冷鞭毛藻株的大亚基（LSU）rDNA 序列与比斯开湾株完全相同，但与加那利群岛株 VGO0775 和澳大利亚株相差 2-3%。此外，两个韩国冷鞭毛藻株的小亚基（SSU）和/或 LSU rDNA 序列与马来西亚株冷鞭毛藻和佛罗里达州株 CCMP1345 和新西兰株 CAWD39（“Coolia monotis”）的序列相差<1%。在基于 LSU rDNA 序列的系统发育树中，韩国冷鞭毛藻株属于包括马来西亚株和这两个株的一个分支。因此，根据系统发育分析，我们认为韩国冷鞭毛藻株与两个大西洋株和澳大利亚株密切相关，而韩国冷鞭毛藻株与马来西亚冷鞭毛藻株以及佛罗里达州和新西兰株有关。
