Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria, BP 901, Hammam-Lif 2050, Tunisia.
Nat Prod Res. 2013;27(2):108-16. doi: 10.1080/14786419.2012.660635. Epub 2012 Feb 16.
In continuation of our search for bioactive secondary metabolites from terrestrial Bacillus spp., a new microbial diketopiperazine, cis-cyclo-(His,Leu) (1) was isolated from the ethyl acetate extract of a strain B. subtilis B38, together with cis-cyclo-(Phe,Phe) (2), tryptophane (3), cis-cyclo-(Leu,Tyr) (4), cis-cyclo-(Trp,Tyr) (5) and macrolactin A (6). The chemical structures of the isolated compounds were identified by comparison of their 1D, 2D NMR and HRESIMS data with authentic spectra and literatures. To the best of our knowledge, this is the first time that cyclo-(His,Leu) has been isolated from natural products.
在继续从陆地芽孢杆菌属中寻找生物活性次生代谢产物的过程中，我们从一株枯草芽孢杆菌 B38 的乙酸乙酯提取物中分离到一种新的微生物二酮哌嗪，顺式-环-(His-Leu)（1），此外还有顺式-环-(Phe-Phe)（2）、色氨酸（3）、顺式-环-(Leu-Tyr)（4）、顺式-环-(Trp-Tyr)（5）和大环内酯 A（6）。通过与真实光谱和文献比较，通过比较其 1D、2D NMR 和 HRESIMS 数据鉴定了分离化合物的化学结构。据我们所知，这是首次从天然产物中分离出环-(His-Leu)。
