Geng Yi-ting, Qiu Jin-rong, Wang Rong, Su Yun-tao, Shu Yong-qian, Yin Yong-mei
Department of Oncology, the First Affiliated Hospital of Nanjing University, Nanjing 210029, China.
Zhonghua Zhong Liu Za Zhi. 2011 Oct;33(10):764-9.
To assess the expression of HER-2 and leptin in gastric cancer and evaluate their relationship with VEGF expression and clinicopathological features, and their prognostic value for gastric cancer patients.
One hundred and ten gastric cancer specimens and the corresponding metastatic lymph nodes were detected for HER-2 by immunohistochemistry (IHC). All primary cancer tissues were detected for leptin, OB-Rb and VEGF. Ninty-six specimens of normal gastric mucosa served as the control.
The expression level of HER-2, leptin and OB-Rb in gastric cancer tissues were significantly higher than those in normal tissues (19.1% vs. 8.0%, 49.1% vs. 34.0%, and 60.9% vs. 46.0%, P < 0.05). HER-2 overexpression was moderately homogenous in primary gastric cancer and matastatic lymph nodes (P = 0.607, Kappa = 0.581). There was a correlation between the expression of HER-2 and leptin, both of which were significantly correlated with tumor invasion depth, metastatic lymph nodes ratio (NR), distal metastasis, TNM stage and VEGF expression. However, there was no significant correlation between OB-Rb expression and the clinicopathological features evaluated. Cox regression multivariate analysis showed that tumor size, histological grade, NR, stage, chemotherapy and HER-2 expression were independent prognostic factors.
HER-2 is stably expressed in primary gastric cancer and metastatic lymph nodes. HER-2 and leptin play an important role in the progression and angiogenesis of gastric cancer. High expression of HER-2 is a prognostic factor for poor outcome.
评估HER-2和瘦素在胃癌中的表达，评价它们与血管内皮生长因子（VEGF）表达及临床病理特征的关系，以及它们对胃癌患者的预后价值。
采用免疫组织化学（IHC）法检测110例胃癌标本及其相应转移淋巴结中的HER-2。检测所有原发性癌组织中的瘦素、长型瘦素受体（OB-Rb）和VEGF。96例正常胃黏膜标本作为对照。
胃癌组织中HER-2、瘦素和OB-Rb的表达水平显著高于正常组织（分别为19.1%对8.0%、49.1%对34.0%、60.9%对46.0%，P<0.05）。HER-2过表达在原发性胃癌和转移淋巴结中呈中度一致性（P = 0.607，Kappa = 0.581）。HER-2与瘦素的表达之间存在相关性，二者均与肿瘤浸润深度、转移淋巴结比例（NR）、远处转移、TNM分期及VEGF表达显著相关。然而，OB-Rb表达与所评估的临床病理特征之间无显著相关性。Cox回归多因素分析显示，肿瘤大小、组织学分级、NR、分期、化疗及HER-2表达是独立的预后因素。
HER-2在原发性胃癌和转移淋巴结中稳定表达。HER-2和瘦素在胃癌进展及血管生成中起重要作用。HER-2高表达是预后不良的一个因素。
