Hikono Hirokazu, Miyazaki Ayako, Mase Masaji, Inoue Makoto, Hasegawa Mamoru, Saito Takehiko
National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Vet Immunol Immunopathol. 2012 Mar 15;146(1):92-6. doi: 10.1016/j.vetimm.2012.01.017. Epub 2012 Jan 26.
Protecting pigs from simultaneous infection with avian, swine, and human influenza viruses would be an effective strategy to prevent the emergence of reassortants with pandemic potential. M2 protein is a candidate antigen for so-called 'universal vaccines,' which confer cross-protection to different influenza viruses in a strain- and subtype-independent manner. We tested whether a recombinant F gene-deleted Sendai virus vector that contained an M2 gene derived from an H5N1 avian influenza virus (SeV/ΔF/H5N1M2) could induce a cross-reactive antibody response to the extracellular domain of M2 protein (M2e) in pigs. SeV/ΔF/H5N1M2 induced an antibody response to M2e when the vector was inoculated intramuscularly. The antibodies induced by SeV/ΔF/H5N1M2 cross-reacted with M2e derived from different avian, swine, and human influenza viruses. In mice, however, SeV/ΔF/H5N1M2 did not confer cross-protection to challenge with a heterologous H3N2 influenza virus. Our results confirm those of other groups indicating that antibodies to M2e do not mediate protection to influenza viruses in pigs.
保护猪免受禽流感、猪流感和人流感病毒的同时感染，将是预防具有大流行潜力的重组病毒出现的有效策略。M2蛋白是所谓“通用疫苗”的候选抗原，这种疫苗能以不依赖毒株和亚型的方式对不同流感病毒提供交叉保护。我们测试了一种重组的、缺失F基因的仙台病毒载体，该载体包含源自H5N1禽流感病毒的M2基因（SeV/ΔF/H5N1M2），是否能在猪体内诱导针对M2蛋白胞外域（M2e）的交叉反应性抗体应答。当通过肌肉注射接种该载体时，SeV/ΔF/H5N1M2诱导了针对M2e的抗体应答。由SeV/ΔF/H5N1M2诱导的抗体与源自不同禽流感、猪流感和人流感病毒的M2e发生交叉反应。然而，在小鼠中，SeV/ΔF/H5N1M2并未对异源H3N2流感病毒的攻击提供交叉保护。我们的结果证实了其他研究小组的结果，表明针对M2e的抗体不能介导对猪流感病毒的保护作用。
