He Yi-lin, Zhang Xiang, Zha Jie, Dai Wen-jun, Luo Feng, Shen Tao
Department of Acute Infectious Disease Control and Prevention, Center for Disease Prevention and Control of Taizhou, Taizhou 225300, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2011 Dec;45(12):1073-6.
To explore the duration of enterovirus (EV) nucleotides positive in feces samples of hand-foot-mouth disease (HFMD) patients after recovery.
A consecutive 6-week follow up were carried out towards 49 cases of laboratory-diagnosed HFMD patients. A total of 5 - 8 g feces sample was collected from each patient once a week. The common EV nucleotides of HFMD were detected by RT-PCR method and analyzed by Kaplan-Meier Survival Analysis Method.
The subtypes of the 49 HFMD patients included 16 enterovirus 71 (EV71), 15 coxsackievirus A16 (CoxA16) and 18 EV; a six-week follow up was carried out among all of them. In the first week, one EV71 patient and two EV patients were lost; in the fourth week, one CoxA16 were lost; and in the fifth week, one EV71 patient was lost. During the consecutive 6-week follow-up, the positive rates of EV nucleotides among EV71 patients were 81.3%, 60.9%, 47.4%, 33.9%, 27.1% and 18.1% separately; and the positive rates in CoxA16 group were 93.3%, 73.3%, 53.3%, 33.3%, 16.7% and 8.3% respectively. In EV group, the positive rates of EV nucleotides were 44.4% and 7.4% in the first two weeks and then turned to negative in the next 4 weeks. There was significant statistical difference in positive rates of EV nucleotides among different patients (χ(2) = 11.78, P = 0.001); however, each group of HFMD patients showed a declined trend with the extension of time.
The duration of EV nucleotides positive in feces samples of HFMD patients lasted for a long period since their recovery; and the positive results in EV71 and CoxA16 patients might last for 6 weeks.
探讨手足口病（HFMD）患者康复后粪便样本中肠道病毒（EV）核酸阳性的持续时间。
对49例实验室确诊的HFMD患者进行连续6周的随访。每周从每位患者收集5 - 8克粪便样本。采用RT-PCR法检测HFMD常见的EV核酸，并通过Kaplan-Meier生存分析法进行分析。
49例HFMD患者的亚型包括16例肠道病毒71型（EV71）、15例柯萨奇病毒A16型（CoxA16）和18例其他EV；对所有患者进行了为期6周的随访。第一周，1例EV71患者和2例其他EV患者失访；第四周，1例CoxA16患者失访；第五周，1例EV71患者失访。在连续6周的随访中，EV71患者中EV核酸的阳性率分别为81.3%、60.9%、47.4%、33.9%、27.1%和18.1%；CoxA16组的阳性率分别为93.3%、73.3%、53.3%、33.3%、16.7%和8.3%。在其他EV组中，前两周EV核酸的阳性率分别为44.4%和7.4%，随后4周转为阴性。不同患者之间EV核酸的阳性率存在显著统计学差异（χ(2)=11.78，P = 0.001）；然而，每组HFMD患者均随时间延长呈下降趋势。
HFMD患者康复后粪便样本中EV核酸阳性持续时间较长；EV71和CoxA16患者的阳性结果可能持续6周。
