Yoshida J
Department of Surgery, University of Illinois, Chicago.
Nihon Geka Gakkai Zasshi. 1990 Aug;91(8):967-71.
Spouting or regeneration of vagus fibers occurs after vagotomy. An axonal tracing method was used to see the long-term effect of serorrhaphy in the prevention of axonal regeneration after proximal gastric vagotomy (PGV) in ferrets.
A neurotracer, wheat-germ agglutinin-horseradish peroxidase (0.5 mg), was injected subserosally at the corpus of the anterior stomach (1) without PGV (n = 4), (2) immediately after PGV (n = 2), (3) one year after PGV with serorrhaphy (n = 3), and (4) one year after PGV without serorrhaphy (n = 3). The ferrets were killed by perfusion-fixation. The brainstem was processed histochemically. Numbers of labeled cells in the dorsal motor nucleus of the vagus were statistically analyzed using square-root transformation.
Numbers of labeled cells were 348 for (1), 13 for (2), 2 for (3), and 43 for (4). Multiple comparisons revealed significant differences in (1) vs. (2), (3), (4) and (3) vs. (4).
The number of preganglionic efferent vagus cells decreased acutely after PGV, which persisted one year. Regenerated vagus axons were less in the chronic group with serorrhaphy than in the group without one. Thus time factor did not influence the regeneration, but serorrhaphy was important in preventing vagal regeneration.
Serorrhaphy during PGV prevents the regeneration of preganglionic efferent vagus fibers.
迷走神经切断术后迷走神经纤维会发生断端出血或再生。采用轴突追踪方法观察缝扎术对雪貂近端胃迷走神经切断术（PGV）后轴突再生的长期影响。
将神经示踪剂麦胚凝集素-辣根过氧化物酶（0.5毫克）浆膜下注射到前胃体部：（1）未行PGV（n = 4）；（2）PGV术后即刻（n = 2）；（3）PGV术后一年且行缝扎术（n = 3）；（4）PGV术后一年未行缝扎术（n = 3）。通过灌注固定处死雪貂。对脑干进行组织化学处理。对迷走神经背运动核中标记细胞的数量进行平方根转换后进行统计学分析。
（1）组标记细胞数量为348个，（2）组为13个，（3）组为2个，（4）组为43个。多重比较显示（1）组与（2）、（3）、（4）组以及（3）组与（4）组之间存在显著差异。
PGV术后节前传出迷走神经细胞数量急性减少，并持续一年。慢性缝扎组再生的迷走神经轴突比未缝扎组少。因此，时间因素不影响再生，但缝扎术对预防迷走神经再生很重要。
PGV术中缝扎可预防节前传出迷走神经纤维再生。
