胆碱酯酶基因表达的调控在体外调节小鼠巨核细胞生成。
Manipulations of cholinesterase gene expression modulate murine megakaryocytopoiesis in vitro.
作者信息
Patinkin D, Seidman S, Eckstein F, Benseler F, Zakut H, Soreq H
机构信息
Department of Biological Chemistry, Hebrew University of Jerusalem, Israel.
出版信息
Mol Cell Biol. 1990 Nov;10(11):6046-50. doi: 10.1128/mcb.10.11.6046-6050.1990.
Megakaryocytopoiesis was selectively inhibited in cultured murine bone marrow cells by a 15-mer oligodeoxynucleotide complementary to the initiator AUG region in butyrylcholinesterase mRNA. Furthermore, conditioned medium from Xenopus oocytes producing recombinant butyrylcholinesterase stimulated megakaryocytopoiesis. These observations implicate butyrylcholinesterase in megakaryocytopoiesis and suggest application of oligodeoxynucleotides for modulating bone marrow development.
通过与丁酰胆碱酯酶mRNA起始AUG区域互补的15聚体寡脱氧核苷酸，在培养的小鼠骨髓细胞中选择性抑制巨核细胞生成。此外，来自产生重组丁酰胆碱酯酶的非洲爪蟾卵母细胞的条件培养基刺激了巨核细胞生成。这些观察结果表明丁酰胆碱酯酶与巨核细胞生成有关，并提示寡脱氧核苷酸在调节骨髓发育中的应用。
Zotero 插件